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Content native Coelenterazine + non-toxic additives for water-solubility Packaging size 500 ug CTZ / vial in a freeze-dried form Application just add sterile water, vortex and use up to 500 ug CTZ in a single injectionCoelenterazine is often dissolved in Propyleneglycol (PG) in combination with other alcohols for in vivo imaging studies. Large intravenous doses of PG given over a short period of time can be toxic, including hyperosmolality, increased anion gap metabolic acidosis (due to lactic acidosis), acute kidney injury, and sepsis-like syndrome.
This can be avoided using water-soluble Coelenterazine. It is formulated to be isosmotic and easily secreted by the kidneys. Thus save for repeated i.v. injections. Water soluble Coelenterazine can be injected up to a 500 µg/100 µl concentration, achieving very high Coelenterazine levels within the body and leading to high Gaussia luciferase signals.
We offer the solubilization vehicle without the Coelenterazine as injection control. Any side effects due to the injection process can be easily monitored, especially in sensitive applications like bioluminescent driven Optogenetics. One vial of the control (Cat. #3031-10+C) contains the same amount of solubilizer as one vial of Cat.#3031. The control will be dissolved in sterile water to create an iso-osmotic solution ready to inject.
To order control: please select ’10 mgs + 10 vials control’ under product options. This includes 20 vials of 500ug water-soluble CTZ and 10 vials of control.
Please inquire if larger quantities of Cat.#3031-10+C are needed.
Protocols & Manuals
Certificates & Data
Luminopsins integrate opto- and chemogenetics by using physical and biological light sources for opsin activation. Berglund K et al.
Proc Natl Acad Sci U S A. 113(3): E358-67 (January 2016)
A Water-Soluble Coelenterazine for Sensitive In Vivo Imaging of Coelenterate Luciferases. Danielle Morse and Bakhos A Tannous
Molecular Therapy 20, 692-693 (April 2012) | doi:10.1038/mt.2012.38