CAT#303 Coelenterazine native

All of our dried substrates are either vacuum sealed or packaged under Argon atmosphere. The substrate in the unopened vessel is stable for at least 3 years if stored at -80ºC. Once dissolved in NanoFuel Solvent it will be stable for at least one year at -20ºC or below. We can’t guarantee the stability in a solvent of your choice (e.g. Ethanol, Methanol), since the auto-oxidation will depend on the water content of the alcohol and its overall quality.

If you are using the recommended amount of 100uM you can run at least 2x 96-well plates with 1 mg of Coelenterazine. This is also including volume that is used to prime the pumps in a luminometer with injection port. 

It will mainly depend on the amount of luciferase present in your assay. You always want to have the substrate in excess. We recommend to use anything between 10 and 100 µM substrate in a buffered solution that will be mixed in equal volumes with your cell culture or protein solution.