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Content 6h-F-Coelenterazine + non-toxic additives for water-solubility Packaging size 500 µg 6h-F-CTZ / vial in a freeze-dried form Application just add sterile water, heat, vortex and use up to 500 µg 6h-F-CTZ in a single injection Package Contents:
500 µgs – 1 vial of 500 µgs lyophilized SOL-6h-F-CTZ
1 mgs – 2 vials of 500 µgs lyophilized SOL-6h-F-CTZ
10 mgs – 20 vials of 500 ugs lyophilized SOL-6h-F-CTZ6h-F-Coelenterazine is often dissolved in Propyleneglycol (PG) in combination with other alcohols for in vivo imaging studies. Large intravenous doses of PG given over a short period of time can be toxic, including hyperosmolality, increased anion gap metabolic acidosis (due to lactic acidosis), acute kidney injury, and sepsis-like syndrome.
This can be avoided using water-soluble 6h-F-Coelenterazine. It is formulated to be isosmotic and easily secreted by the kidneys. Thus save for repeated i.v. injections. Water soluble Coelenterazine can be injected up to a 500 µg/100 µl concentration, achieving very high Coelenterazine levels within the body and leading to high Gaussia luciferase signals.Application Example
Controls
We offer the solubilization vehicle without the Coelenterazine as injection control. Any side effects due to the injection process can be easily monitored, especially in sensitive applications like bioluminescent driven Optogenetics. One vial of the control (Cat. #3481-10+C) contains the same amount of solubilizer as one vial of Cat.#3481. The control will be dissolved in sterile water to create an iso-osmotic solution ready to inject.
To order control: please select ’10 mgs + 10 vials control’ under product options. This includes 20 vials of 500 µg water-soluble 6h-F-CTZ and 10 vials of control.
Please inquire if larger quantities of Cat.#3481-10+C are needed.
Protocols & Manuals
Manual for #3481
Certificates & Data
3481 COA
3481 SDS