- Details & Info
- Protocols & Data
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Kit content
- 50 ml NLuc GLOW buffer for Oplophorus Luciferases
- 1 ml 50x Substrate for Oplophorus Luciferases
Advantages
- one-step assay
- stable luminescent signal (half-life approx. 1.5 hrs)
- affordable
- high sensitivity
- low background, higher S/N ratio
- enough reagent for 1000 assays
- no injection port needed on the luminometer
For a more intense FLASH-type kinetic please see our NLuc FLASH Assay Cat. #324
Protocols & Manuals
Suitable for following luciferases
Oplophorus Luciferase (wildtype, 196 aa, 19.5 kDa) GI:10336559
MAYSTLFIIALTAVVTQASSTQKSNLTFTLADFVGDWQQTAGYNQDQVLEQGGLSSLFQALGVSVTPIQKVVLSGENGLKADIHVIIPYEGLSGFQMGLIEMIFKVVYPVDDHHFKIILHYGTLVIDGVTPNMIDYFGRPYPGIAVFDGKQITVTGTLWNGNKIYDERLINPDGSLLFRVTINGVTGWRLCENILANanoKAZ (171 aa, 19.1 kDa) GI:525342150
MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILANanoLuc® (171 aa, 19.1 kDa) GI:386649645 NanoLuc® is a registered trademark of Promega Corp.
MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILA
Instructions
Certificates & Data
Safety Data Sheet - Buffer
Safety Data Sheet - Substrate
Suggestion
CAT# 381NanoKAZ Luciferase ProteinNanoKAZ Luciferase, an Oplophorus Luciferase mutant is one of the smallest and brightest luciferases. This recombinant ...read morel
CAT# 324NLuc FLASH Assayread morel
CAT# 3815NanoKAZ Luciferase Protein (PBS)NanoKAZ Luciferase in PBS, an Oplophorus Luciferase mutant is one of the smallest and brightest luciferases. This recom...read morel
By Category (NanoKaz (NLuc) Assay)
- 324NLuc FLASH Assay
- 325NLuc GLOW Assay