NanoFuel® GLOW Assay for Oplophorus Luciferases
Introducing a new GLOW Assay kit that will work with Oplophorus Luciferase and its variants NanoKAZ.
- Details & Info
- Protocols & Data
- Related Products
Kit content
- 50 ml NLuc GLOW buffer for Oplophorus Luciferases
- 1 ml 50x Substrate for Oplophorus Luciferases
Advantages
- one-step assay
- stable luminescent signal (half-life approx. 1.5 hrs)
- affordable
- high sensitivity
- low background, higher S/N ratio
- enough reagent for 1000 assays
- no injection port needed on the luminometer
For a more intense FLASH-type kinetic please see our NLuc FLASH Assay Cat. #324
Protocols & Manuals
Suitable for following luciferases
Oplophorus Luciferase (wildtype, 196 aa, 19.5 kDa) GI:10336559
MAYSTLFIIALTAVVTQASSTQKSNLTFTLADFVGDWQQTAGYNQDQVLEQGGLSSLFQALGVSVTPIQKVVLSGENGLKADIHVIIPYEGLSGFQMGLIEMIFKVVYPVDDHHFKIILHYGTLVIDGVTPNMIDYFGRPYPGIAVFDGKQITVTGTLWNGNKIYDERLINPDGSLLFRVTINGVTGWRLCENILANanoKAZ (171 aa, 19.1 kDa) GI:525342150
MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILANanoLuc® (171 aa, 19.1 kDa) GI:386649645 NanoLuc® is a registered trademark of Promega Corp.
MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILA
Instructions
