Coenzyme A Products

Coenzyme A (CoA) free acid is a high-purity, thiol-containing cofactor essential to over 100 enzymatic reactions in cellular metabolism, including fatty acid oxidation, the citric acid cycle, and sterol biosynthesis. Its reactive 3′-phospho-ADP moiety and free thiol group make it the substrate of choice for enzyme kinetics assays, in vitro pathway reconstitution, and metabolic flux studies.

In the field of Bioluminescence CoA has been reported to affect the pattern of light emission in the firefly luciferin–luciferase reaction, and its addition to the assay reagent yields greater enzyme turnover and thus greater luminescence intensity.

Commercial firefly luciferase detection reagents often contain CoASH, and addition of ~100 µM CoASH converts the bioluminescence from a “flash” to a “glow” response — greatly extending the useful measurement window.

Coenzyme A Sodium Salt (CAS 55672-92-9) is a water-soluble cofactor essential for enzymatic acyl-group transfer. Widely used in biochemical research, it supports studies of fatty acid metabolism, energy production, and enzyme activity. In Bioluminescence it helps to produce a stronger, more sustained light signal in Firefly Luciferase Assays. Purity > 98%. For research use only. Not for human or veterinary use.