e-Coelenterazine or Coelenterazine-E (e-CTZ) is a synthetic analogue of native Coelenterazine with an additional ethyl group, forming an additional ring system. First described by Shimomura in 1989.
Renilla muelleri Luciferase shows an increase of 750% in initial intensity and 137% in total light using e-Coelenterazine compared to native Coelenterazine.
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Compound ID e-Coelenterazine or Coelenterazine-E
C28H23N203 CAS# 114496-02-5 IUPAC Name Benz[f]imidazol[1,2-a]quinoxalin-3(6H)-one,5,11-dihydro-8- hydroxy-2-[(4-hydroxyphenyl0methyl]-12-(phenylmethyl) MW 449.5 g/mol
The luciferin is shipped as filtered, lyophilized, batch controlled substrate in 500 µg and 1 mg aliquots (1 mg = 2x 500 µg).
10 mgs is packed as regular powder in one tube.
- pre-aliquoted in small amounts, fresh substrate for every set of experiments
- long shelf-life (packed under Argon)
- faster to dissolve due to its fine crystal structure
- consistent quality between aliquotes
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