e-Coelenterazine or Coelenterazine-E (e-CTZ) is a synthetic analogue of native Coelenterazine with an additional ethyl group, forming an additional ring system. First described by Shimomura in 1989. Renilla muelleri Luciferase shows an increase of 750% in initial intensity and 137% in total light using e-Coelenterazine compared to native Coelenterazine. Also available as water-soluble form (Cat.# 3551) for in vivo experiments.
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e-Coelenterazine Details
Compound ID e-Coelenterazine or Coelenterazine-E Molecular Formula
C28H23N203 CAS# 114496-02-5 IUPAC Name Benz[f]imidazol[1,2-a]quinoxalin-3(6H)-one,5,11-dihydro-8- hydroxy-2-[(4-hydroxyphenyl0methyl]-12-(phenylmethyl) MW 449.5 g/mol We recommend using CAT#399 NanoFuel Solvent for dissolving and stabilizing native Coelenterazine.
The luciferin is shipped as filtered, lyophilized, batch controlled substrate in 500 µg and 1 mg aliquots (1 mg = 2x 500 µg).
10 mgs is packed as regular powder in one tube.
Advantages
- pre-aliquoted in small amounts, fresh substrate for every set of experiments
- long shelf-life (packed under Argon)
- faster to dissolve due to its fine crystal structure
- consistent quality between aliquotes
Protocols & Manuals
e-CTZ Manual
Certificates & Data
Certificate of Analysis
Safety Data Sheet
Suggestion
CAT# 399NanoFuel SolventNanoFuel® Solvent is NanoLight’s solution to the problem associated with the solubility of Coelenterazine and the man...read morel
CAT# 355-INJe-Coelenterazine-INJe-CTZ is now available in sterile injection vials with a low retention volume. Each kit is suppled with two 'Inject-A-...read morel
By Category (Coelenterazines)
