A substrate for Renilla luciferases (Rluc) but not Gaussia luciferase. It is used for real-time detection of calcium ions, gene reporter assays and a favored substrate for BRET. This derivative has an emission maximum around 400 nm and therefore minimal interference with the emission of the GFP acceptor.
Coelenterazine 400a is also know under the Perkin Elmer trademark DeepBlueC™.
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Compound ID Coelenterazine 400a, 1-bisdeoxycoelenterazine, di-dehydrocoelenterazine, DeepBlueCTM
C26H21N3O CAS# 70217-82-2 IUPAC Name 2,8-DIBENZYL-6-PHENYL-IMIDAZO[1,2A]PYRAZIN-3-(7H)- 1; BISDEOXYCOELENTERAZINE MW 391.5 g/mol
- pre-aliquoted in small amounts, fresh substrate for every set of experiments
- long shelf-life (packed under Argon)
- faster to dissolve due to its fine crystal structure
- consistent quality between aliquotes
The luciferin is shipped as filtered, lyophilized, batch controlled substrate
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