Prolume Purple or methoxy e-Coelenterazine (Me-O-e-CTZ) is a new synthetic analogue of e-Coelenterazine with an additional methoxy group quenching the emission maximum from 475 nm to 405 nm. Nanolight™ Technologies developed Prolume Purple. (US Patent 2013/057660)
Prolume Purple exhibits an up to 13-fold higher luminescence than CTZ400a (“deep blue C”).
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Compound ID Prolume Purple (PP-CTZ) Molecular Formula C29H25N3O3 Alternative Name methoxy e-Coelenterazine (me-O-eCTZ) MW 463.53 Application emits around 405 nm with RLuc, RLucII, RLuc3, RLuc8 and Green Renilla Luc useful as a substrate in bioluminescence imaging (BLI) with near-infrared BRET partners. Solvent We recommend NanoFuel Solvent. Low solubility in Ethanol Comparison to CTZ400a
Emission spec with Renilla Luciferases
The advantages of lyophilized luciferins are:
- pre-aliquoted in small amounts, fresh substrate for every set of experiments
- long shelf-life (packed under Argon)
The luciferin is shipped as filtered, lyophilized, batch controlled substrate
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CAT# 340Coelenterazine 400aA substrate for Renilla luciferases (Rluc) but not Gaussia luciferase. It is used for real-time detection of calcium io...read morel
CAT# 399NanoFuel Solventread morel
CAT# 3692Prolume Purple- SOL (in vitro)Methoxy e-Coelenterazine (Prolume Purple) formulated to be water-soluble for in vitro applications with Renilla Lucifera...read morel
By Category (Prolume Purple (methoxy e-CTZs))
- 369Prolume Purple
- 367Prolume Purple II
- 365Prolume Purple III
- 363Prolume Purple IV
- 361Prolume Purple V
- 360Prolume Purple Sampler
- 398NanoFuel-V Solvent
- 399NanoFuel Solvent
- 3692Prolume Purple- SOL (in vitro)
