Prolume Purple or methoxy e-Coelenterazine (Me-O-e-CTZ) is a new synthetic analogue of e-Coelenterazine with an additional methoxy group quenching the emission maximum from 475 nm to 405 nm. Nanolight™ Technologies developed Prolume Purple. (US Patent 2013/057660)
Prolume Purple exhibits an up to 13-fold higher luminescence than CTZ400a (“deep blue C”).
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Compound ID Prolume Purple (PP-CTZ) Molecular Formula C29H25N3O3 Alternative Name methoxy e-Coelenterazine (me-O-eCTZ) MW 463.53 Application emits around 405 nm with RLuc, RLucII, RLuc3, RLuc8 and Green Renilla Luc useful as a substrate in bioluminescence imaging (BLI) with near-infrared BRET partners. Solvent We recommend NanoFuel Solvent. Low solubility in Ethanol
Comparison to CTZ400a
Emission spec with Renilla Luciferases
The advantages of lyophilized luciferins are:
- pre-aliquoted in small amounts, fresh substrate for every set of experiments
- long shelf-life (packed under Argon)
The luciferin is shipped as filtered, lyophilized, batch controlled substrate
Protocols & Manuals
Certificates & Data
Near-infrared bioluminescent proteins for two-color multimodal imaging. Rumyantsev KA, Turoverov KK, Verkhusha VV. Nature Sci Rep. 2016 Nov 11; PMID: 27833162.
Comparison of PP-I to IV as substrates for a NIR BRET construct in bioluminescent imaging.
Monitoring G protein-coupled receptor and β-arrestin trafficking in live cells using enhanced bystander BRET. Namkung Y, Le Gouill C, Lukashova V, Kobayashi H, Hogue M, Khoury E, Song M, Bouvier M, Laporte SA. Nat Commun. 2016 Jul PubMed PMID: 27397672
Prolume Purple (Cat. #369) was enabling these researches to follow protein movement within a single cell in real-time for up to 20 min using a sophisticated BRET system.
Bioluminescence Assisted Switching and Fluorescence Imaging (BASFI)
Luyuan Zhang, Fang Xu, Zhixing Chen, Xinxin Zhu and Wei Min. J.Phys. Chem. Lett., 2013, 4, pp 3897-3902
Good overview on spectral properties of the Prolume Purple substrates.