Methoxy-eCoelenterazine-F (Me-O-eCTZ-F) is a new synthetic analogue of e-Coelenterazine-F with an additional methoxy groups emitting at 410nm with Green Renilla Luciferase. Nanolight™ Technologies developed Prolume Purple III. (US Patent 2013/057660)
If you are using RLuc8 or wildtyp Renilla Luc please use Prolume Purple (Cat.# 369).
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Compound ID Prolume Purple III (PP-II CTZ) Molecular Formula C29H24FN3O2 Alternative Name Methoxy-eCoelenterazine-F (Me-O-eCTZ-F) MW 465.5 g/mol Solvent We recommend NanoFuel Solvent. Low solubility in Ethanol
Activity compared to other CTZs
Emission specs with Green RLuc
The advantages of lyophilized luciferins are
The luciferin is shipped as filtered, lyophilized, batch controlled substrate for 250µg and 1mg (4x 250µg) aliquots.10 mgs are packed as regular powder in one tube.
- pre-aliquoted in small amounts, fresh substrate for every set of experiments
- long shelf-life (packed under Argon)
- faster to dissolve due to its fine crystal structure
- consistent quality between aliquotes
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