Methoxy e-Coelenterazine (Prolume Purple) formulated to be water-soluble for in vitro applications with Renilla Luciferase.
Prolume Purple exhibits an up to 13-fold higher luminescence than CTZ400a (“deep blue C”).
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Content Methoxy e-Coelenterazine + non-toxic additives for water-solublity. Packaging Size adding 10ml of buffer will give a 50 μM solution, adding 5 ml buffer will give a 100 μM solution. Application Just add your buffer, vortex and use with for example in BRET assays
For details about the properties of Prolume Purple please refer to the information listed for Prolume Purple (Cat.#369) . The emission maximum with Renilla Luciferase is 405 nm thus emitting a purple light. Nanolight™ Technologies developed Prolume Purple. Patent pending.
Advantages of water-soluble Coelenterazine
- convenient to use, no pre-dissolving of h CTZ in alcohol necessary
- can be used with different buffers for assay optimization
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Prolume Purple (Cat. #369) was enabling these researches to follow protein movement within a single cell in real-time for up to 20 min using a sophisticated BRET system:
Monitoring G protein-coupled receptor and beta-arrestin trafficking in live cells using enhanced bystander BRET Yoon Namkung, Christian Le Gouill, Viktoria Lukashova, Hiroyuki Kobayashi, Mireille Hougue, Etienne Khoury, Mideum Song, Micharl Bouvier, Stephane A. Laporte