CAT#345 Coelenterazine F

product picture of Coelenterazine-F

Coelenterazine F is the preferred substrate for Aequorin and used as based calcium indicator. When it reconstitutes apoaequorin into functional aequorin, it produces roughly 20-fold higher luminescence intensity than native coelenterazine does at the same (100 nM) Calcium concentration, with emission shifted slightly to the red (~472–473 nm vs. ~465 nm for native). Recommended when high Calcium sensitivity of regenerated Aequorin is needed. We recommend using CAT#399 NanoFuel Solvent for dissolving and stabilizing native Coelenterazine.