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Content h-Coelenterazine + non-toxic additives for water-solubility Packaging size 500 µg h-CTZ / vial in a freeze-dried form Application just add sterile water, vortex and use up to 500 µg CTZ in a single injectionCoelenterazine compounds are often dissolved in Propyleneglycol (PG) in combination with other alcohols for in vivo imaging studies. Large intravenous doses of PG given over a short period of time can be toxic, including hyperosmolality, increased anion gap metabolic acidosis (due to lactic acidosis), acute kidney injury, and sepsis-like syndrome.
This can be avoided using water-soluble h-Coelenterazine. It is formulated to be isosmotic and easily secreted by the kidneys. Thus save for repeated i.v. injections. Water-soluble h-Coelenterazine can be injected up to a 500 µg/100 µl concentration, achieving very high Coelenterazine levels within the body and leading to high luciferase signals.h-Coelenterazine is one of the preferred substrates for Renilla Luciferase.If you use Gaussia Luciferase in your assay please use Cat. #3031
Advantages of water-soluble Coelenterazine
- non-toxic, iso-osmotic, Propylene-Glycol free, Alcohol free
- long shelf-life, sealed under vacuum
- dissolves fast in sterile water
- low viscosity – ideal for injections
- consistent quality due to small aliquot sizes
We offer the solubilization vehicle without the h-Coelenterazine as injection control. Any side effects due to the injection process can be easily monitored, especially in sensitive applications like bioluminescent driven Optogenetics. One vial of the control (Cat. #3011-10+C) contains the same amount of solubilizer as one vial of Cat.#3011. The control will be dissolved in sterile water to create an iso-osmotic solution ready to inject.
To order control: please select ’10 mgs + 10 vials control’ under available sizes. This includes 20 vials of 500 µg water-soluble h-CTZ and 10 vials of control.
Please inquire if larger quantities of Cat.#3011-10+C are needed.
Protocols & Manuals
Certificates & Data
A detailed describtion of this new water soluble Coelenterazine (in combination with Gaussia Luciferase) can be found in:
A Water-Soluble Coelenterazine for Sensitive In Vivo Imaging of Coelenterate
Luciferases. Danielle Morse and Bakhos A Tannous
Molecular Therapy 20, 692-693 (April 2012) | doi:10.1038/mt.2012.38