v-Coelenterazine is an analogue of Coelenterazine that causes a shift in the emission-spectrum to longer wavelengths (515nm and above). Renilla Luciferase is the preferred catalyst for this compound. For more details see here.
- Details & Info
- Protocols & Data
Content v Coelenterazine + additives for water-solubility Packaging Size adding 10ml buffer will give a 50µM solution, adding 5 ml buffer will give a 100µM solution. Application Just add an aequeous buffer of your choice (e.g. PBS), invert a few times and use for luminometer assays. Luciferase Renilla Luiferase and its mutated versions (RLuc2, RLuc8, Green Renilla Luciferase)
Advantages of water-soluble Coelenterazine
- convenient to use, no pre-dissolving of h CTZ in alcohol necessary
- can be used with different buffers for assay optimization
Protocols & Manuals