e-CTZ is now available in sterile injection vials with a low retention volume. Each kit is suppled with two ‘Inject-A-Lume’ vials of 500 ug e-CTZ and 500 ul of steril ‘Fuel-Inject’ diluent. It was reported that this analogue enhances the signal by two-fold in Renilla Luciferase in vivo studies.
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Compound ID e-Coelenterazine or Coelenterazine-E Molecular Formula C28H23N203 CAS# 114496-02-5 IUPAC Name Benz[f]imidazol[1,2-a]quinoxalin-3(6H)-one,5,11-dihydro-8- hydroxy-2-[(4-hydroxyphenyl0methyl]-12-(phenylmethyl) MW 449.5 g/mol
e-coelenterazine or Coelenterazine-E (e-CTZ) is a synthetic analogue of native Coelenterazine with an additional ethyl group, forming an additional ring system. First described by Shimomura in 1989.
For Renilla muelleri Luciferase, an increase of 750% in initial intensity and 137% in total light was observed using e-Coelenterazine compared to naitve Coelenterazine.
Note: In will not be utilized by Gaussia Luciferase.
- 2x 500 µg Inject-A-Lume e-Coelenterazine
- 1x 500 µl Fuel-Inject (substrate solvent)
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