Prolume Purple II or Methoxy-Coelenterazine-Methoxy (Me-O-CTZ-O-Me) is a new synthetic analogue of Coelenterazine with two additional methoxy groups emitting at 400nm with Renilla Luciferase 8 (RLuc8) and Green Renilla Luciferase. Nanolight™ Technologies developed Prolume Purple II (US Patent 2013/057660).
Prolume Purple II exhibits an up to 5-fold higher luminescence than CTZ400a (“deep blue C”).
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Molecular Formula C28H25N3O3 Alternative Name Methoxy-Coelenterazine-Methoxy (Me-O-CTZ-O-Me) MW 451.5 g/mol Solvent We recommend NanoFuel Solvent. Low solubility in Ethanol
Activity compared to CTZ-400a
Emission specs with RLuc8
The advantages of lyophilized luciferins are:
- pre-aliquoted in small amounts, fresh substrate for every set of experiments
- long shelf-life (packed under Argon)
- faster to dissolve due to its fine crystal structure
- consistent quality between aliquotes
The luciferin is shipped as filtered, lyophilized, batch controlled substrate
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