Prolume Purple IV or Methoxy-Coelenterazine-Iodine (Me-O-CTZ-I) is a new synthetic analogue of Coelenterazine-I with an additional methoxy group. So far we were not able to determine a luciferase that is working with this unique compound. Activity with RLuc8, Rluc3 and Green Renilla Luciferase were very low.
Nanolight™ Technologies developed Prolume Purple IV. (US Patent 2013/057660).
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Compound ID Prolume Purple IV (PP-IV CTZ) Molecular Formula C27H22IN3O2 Alternative Name Methoxy-Coelenterazine-Iodine (Me-O-CTZ-I) MW 547.4 g/mol Solvent We recommend NanoFuel Solvent. Low solubility in Ethanol
The luciferin is shipped as filtered, lyophilized, batch controlled substrate for 250µg and 1mg (4x 250µg) aliquots.10 mgs are packed as regular powder in one tube.
- pre-aliquoted in small amounts, fresh substrate for every set of experiments
- long shelf-life (packed under Argon)
- faster to dissolve due to its fine crystal structure
- consistent quality between aliquots
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