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e-Coelenterazine-SOL (in vivo) Details
Content e-Coelenterazine + non-toxic additives for water-solubility Packaging size 500 ug e-CTZ / vial in a freeze-dried form Application just add sterile water, vortex and use up to 500 ug e-CTZ in a single injection Package Contents:
500 ugs – 1 vial of 500 ugs lyophilized e-CTZ-SOL
1 mgs – 2 vials of 500 ugs lyophilized e-CTZ-SOL
10 mgs – 20 vials of 500 ugs lyophilized e-CTZ-SOLe-Coelenterazine is often dissolved in Propyleneglycol (PG) in combination with other alcohols for in vivo imaging studies. Large intravenous doses of PG given over a short period of time can be toxic, including hyperosmolality, increased anion gap metabolic acidosis (due to lactic acidosis), acute kidney injury, and sepsis-like syndrome.
This can be avoided using water-soluble e-Coelenterazine. It is formulated to be isosmotic and easily secreted by the kidneys. Thus save for repeated i.v. injections. Water soluble e-Coelenterazine can be injected up to a 500 µg/100 µl concentration, achieving very high e-Coelenterazine levels within the body and leading to high Renilla luciferase signals.Application Example
Controls
We offer the solubilization vehicle without the e-Coelenterazine as injection control. Any side effects due to the injection process can be easily monitored. One vial of the control (Cat. #3551-10+C) contains the same amount of solubilizer as one vial of Cat.#3551. The control will be dissolved in sterile water to create an iso-osmotic solution ready to inject.
To order control: please select ’10 mgs + 10 vials control’ under product options. This includes 20 vials of 500ug water-soluble CTZ and 10 vials of control.
Please inquire if larger quantities of Cat.#3551-10+C are needed.
Protocols & Manuals
Manual #3551
Certificates & Data
COA #3551
SDS #3551