The NanoFuel® Assay kit for Firefly Luciferase kit provides enough buffer for approx. 1000 assays. Optimized buffer conditions will give you the best signal over a half-life of 45 min. Therefore no injection luminometer is required. This kit can be used for high-throughput screenings due to its steady signal.
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FLuc Assay Kit content
- 50 ml FLuc Buffer – provides optimal reaction conditions
- 1 vial Luciferin substrate (please add to buffer before use)
Properties of FLuc Assay Buffer
- creates optimal conditions for FLuc
- low background, thus higher S/N ratio
- very high and steady signal
- can be used in high-throughput experiments
- NOTE: this buffer does not contain any detergent,
intracellular expressed FLuc will require lysis - for intracellular assays a 5x Universal Lysis Buffer (Cat.#333) can be purchased, please select “add lysis” under product options
Protocols & Manuals
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Quick Instructions
Certificates & Data
#318_FLuc_Assay_quick_instructions
318_Firefly Luciferase Assay Reagent_SDS_06-2020
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