Luciferase Proteins Products
| Cat# | Name | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 321 | One of the smallest and brightest cloned luciferases. | 
One of the smallest and brightest cloned luciferases.
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| 371 | Gaussia(M2)-Luciferase-Streptavidin is a conjugate of one of ... | 
Gaussia(M2)-Luciferase-Streptavidin is a conjugate of one of the brightest luciferases and a protein with the highest known affinity in nature. This fusion protein can be used to detect trace amounts of biotinylated protein, DNA, or other biotinylated bio-molecules.
In experiments we were able to visualize the GLuc luminescence on the surface of Agarose-Biotin beads using a standard light microscope in total darkness. GLuc-SA was engineered by connecting one molecule of GLuc to one Streptavidin subunit. Streptavidin consists of 4 subunits with theoretically 4 biotin binding sites. Purified Streptavidin will have the ability to bind between 3.2 and 3.9 biotin molecules. Due to extensive purification steps, no free Streptavidin is present and cross-linking will be prevented, enabling quantitative read-outs in luminescent assays. 
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| 381 | NanoKAZ Luciferase, an Oplophorus Luciferase mutant is one o ... | 
NanoKAZ Luciferase, an Oplophorus Luciferase mutant is one of the smallest and brightest luciferases. This recombinant protein was affinity purified to give it a maximum specific activity. Patent US 9,315,783 and US 9,404,145. |
| Compound ID | NanoKAZ Luciferase (NLuc) enzyme |
| MW | 19.2 kDa (theoretical) |
| Appearance | clear liquid as 1 mg/ml in TBS |
| Applications | as positive control in luminometer assays and general assay optimization |
| Production | recombinant expression in E.coli with subsequent affinity purification |
Sequence
MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILA
SDS-PAGE
Activity
NanoKAZ Luciferase in PBS, an Oplophorus Luciferase mutant is one of the smallest and brightest luciferases. This recombinant protein was affinity purified to give it a maximum specific activity. Buffered in PBS for convenient linkage chemistry where Tris (or other primary amines) would interfere.
Patent US 9,315,783 and US 9,404,145.
| Compound ID | NanoKAZ Luciferase (NLuc) enzyme (PBS) |
| MW | 19.2 kDa (theoretical) |
| Appearance | clear liquid as 1mg/ml in TBS |
| Applications | as positive control in luminometer assays and general assay optimization |
| Production | recombinant expression in E.coli with subsequent affinity purification |
Sequence
MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILA
SDS-PAGE
Activity
95% purity (recombinant). Supplied as lyophilized powder.
ABS280 of 0.63 = 1.0 mg RM Luc
MTSKVYDPELRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENAVIFLHGNAASSYLWRHVVPHVEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTEWFKHLNLPKKIIFVGHDWGACLAFHYCYEHQDRIKAVVHAESVVDVIESWDEWPDIEEDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREIPLVKGGKPDVVEIVRNYNAYLRASHDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVKGLHFSQEDAPDEMGNYIKSFVERVLKNEQ
