Luciferins & Substrates (NanoFuel)

Assay Reagents

Luciferases & GFPs

Antibodies

Vectors (NanoLights)

Coelenterazines Products

Cat#Name
CAT#303(c).product-thumb 303
>95% pure, native Coelenterazine. lyophilized.
CAT#303(c).product-thumb

Coelenterazine Free Base purity >95%

The most abundant form of Coelenterazine found in nature.
Native Coelenterazine is the preferred substrate for Gaussia luciferase.

 

We recommend using CAT#399 NanoFuel Solvent for dissolving and stabilizing native Coelenterazine.

Coelenterazine native Structure
Compound IDnative Coelenterazine

Molecular Formula

C26H21N3O3
CAS#55779-48-1
IUPAC Name8-benzyl-6-(4-hydroxyphenyl)-2-[(4-hydroxyphenyl)methyl] -7H-imidazo [1,2-a]pyrazin-3-one
MW423.46 g/mol

Package Contents:

500 ugs  – 1 vial of 500 ugs lyophilized CTZ
1 mgs – 2 vials of 500 ugs lyophilized CTZ
5 mgs – 1 vial of 5 mgs lyophilized CTZ
10 mgs – 2 vials of 5 mgs lyophilized CTZ

The advantages of lyophilized luciferins are:

  • pre-aliquoted in small amounts, fresh substrate for every set of experiments
  • long shelf-life (packaged under Argon)
  • faster to dissolve due to its fine crystal structure
  • consistent quality between aliquots

This luciferin is shipped as filtered, lyophilized, batch controlled substrate.
Available in different sizes. Please inquire for custom aliquots.

 

 

CAT-301d.product-thumb 301
A 2-deoxy derivative of native Coelenterazine. Up to 20-f ...
CAT-301d.product-thumb

A 2-deoxy derivative of native Coelenterazine.

Up to 20-fold higher initial light-output than native Coelenterazine with Apoaequorin.
Reduced but longer lasting kinetic with Renilla luciferase.

 

We recommend using CAT#399 NanoFuel Solvent for dissolving and stabilizing native Coelenterazine.

h-CTZ
Compound IDCoelenterazine h or 2-Deoxycoelenterazine

Molecular Formula

C26H21N3O2
CAS#50909-86-9
IUPAC Name2,8-dibenzyl-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one
MW407.5 g/mol

Package Contents:

500 ugs  – 1 vial of 500 ugs lyophilized CTZ-h
1 mgs – 2 vials of 500 ugs lyophilized CTZ-h
5 mgs – 1 vial of 5 mgs lyophilized CTZ-h
10 mgs – 2 vials of 5 mgs lyophilized CTZ-h

 

The advantages of lyophilized luciferins are:

  • Pre-aliquoted in small amounts, fresh substrate for every set of experiments
  • long shelf-life (packed under Argon)
  • faster to dissolve due to its fine crystal structure
  • consistent quality between aliquotes

The luciferin is shipped as filtered, lyophilized, batch controlled substrate

CAT-340b.product-thumb 340
A substrate for Renilla luciferases (Rluc) but not Gaussia ...
CAT-340b.product-thumb

A substrate for Renilla luciferases (Rluc) but not Gaussia luciferase. It is used for real-time detection of calcium ions, gene reporter assays and a favored substrate for BRET. This derivative has an emission maximum around 400 nm and therefore minimal interference with the emission of the GFP acceptor.
Coelenterazine 400a is also know under the Perkin Elmer trademark DeepBlueC™.

 

We recommend using CAT#397 NanoFuel-400a Solvent for dissolving and stabilizing Coelenterazine 400a.

340 - structure
Compound IDCoelenterazine 400a, 1-bisdeoxycoelenterazine, di-dehydrocoelenterazine, DeepBlueCTM

Molecular Formula

C26H21N3O
CAS#70217-82-2
IUPAC Name2,8-DIBENZYL-6-PHENYL-IMIDAZO[1,2A]PYRAZIN-3-(7H)- 1; BISDEOXYCOELENTERAZINE
MW391.5 g/mol

Package Contents:

500 ugs  – 1 vial of 500 ugs lyophilized CTZ 400a
1 mgs – 2 vials of 500 ugs lyophilized CTZ 400a
5 mgs – 1 vial of 5 mgs lyophilized CTZ 400a
10 mgs – 2 vials of 5 mgs lyophilized CTZ 400a

 

Advantages

  • pre-aliquoted in small amounts, fresh substrate for every set of experiments
  • long shelf-life (packed under Argon)
  • faster to dissolve due to its fine crystal structure
  • consistent quality between aliquotes

The luciferin is shipped as filtered, lyophilized, batch controlled substrate

CAT-355a.product-thumb 355
e-Coelenterazine or Coelenterazine-E (e-CTZ) is a synthetic ...
CAT-355a.product-thumb

e-Coelenterazine or Coelenterazine-E (e-CTZ) is a synthetic analogue of native Coelenterazine with an additional ethyl group, forming an additional ring system. First described by Shimomura in 1989.

Renilla muelleri Luciferase shows an increase of 750% in initial intensity and 137% in total light using e-Coelenterazine compared to native Coelenterazine.

eCTZ for webpage
Compound IDe-Coelenterazine or Coelenterazine-E

Molecular Formula

C28H23N203
CAS#114496-02-5
IUPAC NameBenz[f]imidazol[1,2-a]quinoxalin-3(6H)-one,5,11-dihydro-8- hydroxy-2-[(4-hydroxyphenyl0methyl]-12-(phenylmethyl)
MW449.5 g/mol

We recommend using CAT#399 NanoFuel Solvent for dissolving and stabilizing native Coelenterazine.

 

The luciferin is shipped as filtered, lyophilized, batch controlled substrate in 500 µg and 1 mg aliquots (1 mg = 2x 500 µg). 

10 mgs is packed as regular powder in one tube.

Advantages

  • pre-aliquoted in small amounts, fresh substrate for every set of experiments
  • long shelf-life (packed under Argon)
  • faster to dissolve due to its fine crystal structure
  • consistent quality between aliquotes

 

 

 

nl-product-placeholder 345
Coelenterazine F is the preferred substrate for Aequorin ...
nl-product-placeholder

Coelenterazine F is the preferred substrate for Aequorin

 

We recommend using CAT#399 NanoFuel Solvent for dissolving and stabilizing native Coelenterazine.

CTZ-F
Compound IDCoelenterazine-Fluoride or Coelenterazine F or CTZ-F

Molecular Formula

C26H20FN3O2
CAS#123437-16-1
IUPAC Name8-benzyl-2-(4-fluorobenzyl)-6-(4-hydroxyphenyl)imidazo[1,2- a]pyrazin-3(7H)-one
MW425.5 g/mol

Package Contents:

500 ugs  – 1 vial of 500 ugs lyophilized CTZ-f
1 mgs – 2 vials of 500 ugs lyophilized CTZ-f
5 mgs – 1 vial of 5 mgs lyophilized CTZ-f
10 mgs – 2 vials of 5 mgs lyophilized CTZ-f

 

Advantages

  • pre-aliquoted in small amounts, fresh substrate for every set of experiments
  • long shelf-life (packed under Argon)
  • faster to dissolve due to its fine crystal structure
  • consistent quality between aliquotes

 

The luciferin is shipped as filtered, lyophilized, batch controlled substrate

nl-product-placeholder 346
e-Coelenterazine-F  purity >95% is a new Coelenterazine ...
nl-product-placeholder

e-Coelenterazine-F  purity >95% is a new Coelenterazine derivative utilized by Renilla luciferase.

 

We recommend using CAT#399 NanoFuel Solvent for dissolving and stabilizing native Coelenterazine.

eCTZf
Compound IDeCoelenterazine-F

Molecular Formula

C28H22FN3O2
CAS#not registered
MW451.49 g/mol

Emission spectrum of eCTZ-F

eCTZ-F is showing a unique emission spectrum in combination with Renilla Luciferase (RLuc), emitting a high energy wide spectrum from 400 to 550 nm. Please see the manual for details.

The luciferin is shipped as filtered, lyophilized, batch controlled substrate in 500 µg and 1 mg aliquots (1 mg = 2x 500 µg). 

10 mgs is packed as regular powder in one tube.

Advantages

  • pre-aliquoted in small amounts, fresh substrate for every set of experiments
  • long shelf-life (packed under Argon)
  • faster to dissolve due to its fine crystal structure
  • consistent quality between aliquots

 

 

CAT-399.product-thumb 397
NanoFuel®-400a Solvent is NanoLight’s solution to the pro ...
CAT-399.product-thumb

NanoFuel®-400a Solvent is NanoLight’s solution to the problem associated with the solubility of the highly hydrophobic Coelenterazine 400a.

Coelenterazine-400a (DeepBlueC™) can be dissolved up to a concentration of 2.5 mg/ml.

NanoFuel-400a Solvent also guarantees Coelenterazine 400a stability if store at -20°C for a few weeks or at -80°C for up to one year.

1 ml / tube or 10 ml / tube

Optimized for IN VITRO use with the following Coelenterazine analog:

    CAT-370b.product-thumb 370
    V-Coelenterazine (V-CTZ) is a synthetic analogue of Coelent ...
    CAT-370b.product-thumb

    V-Coelenterazine (V-CTZ) is a synthetic analogue of Coelenterazine with an additional Benzyl ring. This compound has the unique ability to shift the emission spectra of wildtyp Renilla Luciferase, RLuc8 and Green Renilla Luciferase to longer wavelengths.

     

    V-Coelenterazine is particular difficult to get into solution. We recommend using CAT#398 NanoFuel-V Solvent for dissolving and stabilizing v-Coelenterazine.

    vCTZ-structure
    Compound IDv-Coelenterazine (V-CTZ) or Coelenterazine-v

    Molecular Formula

    		C28H21N3O3
    Alternative Name16-benzyl-5-hydroxy-13-[(4-hydroxyphenyl)methyl]-11,14,17-triazatetracyclo [8.7.0.0^{2,7}.0^{11,15}] heptadeca-1(10),2(7),3,5,8,13,15-heptaen-12-one
    MW447.48 g/mol
    SolventWe recommend NanoFuel-V Solvent, especially designed for this compound. Solubility in Ethanol or Methanol is very low.

    Shift in emission with RLuc 8

    v-CTZ-spectrum

    Renilla Luciferases emission spectra

    v-CTZ-emissions

    The advantages of lyophilized luciferins

    • pre-aliquoted in small amounts, fresh substrate for every set of experiments
    • long shelf-life (packed under Argon)
    • faster to dissolve due to its fine crystal structure
    • consistent quality between aliquotes

     

    The luciferin is shipped as filtered, lyophilized, batch controlled substrate in 500 µg and 1 mg aliquots (1 mg = 2x 500 µg). 

    10 mgs is packed as regular powder in one tube.

    CAT-399.product-thumb 398
    Designed by NanoLight to increase the solubility of v-Coele ...
    CAT-399.product-thumb

    Designed by NanoLight to increase the solubility of v-Coelenterazine and Methoxy v-Coelenterazine-Methoxy. Both compounds have a very low solubility in Ethanol and Methanol. Using NanoFuel-V solvent gives you v-CTZ concentrations of up to 4 mg/ml.

    1 ml/tube

    Optimized for IN VITRO use with following Coelenterazine analogs:

    CAT#370 v-Coelenterazine

    CAT#361 Prolume Purple V (Methoxy-v-Coelenterazine-Methoxy)

     

    It is not designed for IN VIVO use.

    CAT-399.product-thumb 399
    NanoFuel® Solvent is NanoLight’s solution to the problem ...
    CAT-399.product-thumb

    NanoFuel® Solvent is NanoLight’s solution to the problem associated with the solubility of Coelenterazine and the many new Coelenterazine analogs that have been made available in recent years. 

    Native Coelenterazine can be dissolved in a 10 times higher concentration compared to other alcohols.

    NanoFuel-Solvent also guarantees Coelenterazine stability if store at -20°C for a few weeks or at -80°C for up to one year.

    1 ml / tube

    Optimized for IN VITRO use with our following Coelenterazine analogs .

     

    Not Designed for In Vivo use

    For live animal imaging, please use Inject-A-Fuel or Water Soluble CTZs: