In-Vivo Products Products
| Cat# | Name | |||||||||||||
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| 303-INJ | Injectable native Coelenterazine, highly pure (99.7%) and op ... | 
Injectable native Coelenterazine, highly pure (99.7%) and optimized for in vivo research.
Recommended path of injection: intra-peritoneal cavity. For animal use only. | |||||||||||
| 3031 | Native Coelenterazine formulated to be water-soluble and saf ... | 
Native Coelenterazine formulated to be water-soluble and safe for in vivo imaging applications with Gaussia Luciferase.
Package Contents: 500 ugs – 1 vial of 500 ugs lyophilized SOL-CTZ
Coelenterazine is often dissolved in Propyleneglycol (PG) in combination with other alcohols for in vivo imaging studies. Large intravenous doses of PG given over a short period of time can be toxic, including hyperosmolality, increased anion gap metabolic acidosis (due to lactic acidosis), acute kidney injury, and sepsis-like syndrome.
This can be avoided using water-soluble Coelenterazine. It is formulated to be isosmotic and easily secreted by the kidneys. Thus save for repeated i.v. injections. Water soluble Coelenterazine can be injected up to a 500 µg/100 µl concentration, achieving very high Coelenterazine levels within the body and leading to high Gaussia luciferase signals. Application Example
ControlsWe offer the solubilization vehicle without the Coelenterazine as injection control. Any side effects due to the injection process can be easily monitored, especially in sensitive applications like bioluminescent driven Optogenetics. One vial of the control (Cat. #3031-10+C) contains the same amount of solubilizer as one vial of Cat.#3031. The control will be dissolved in sterile water to create an iso-osmotic solution ready to inject. To order control: please select ’10 mgs + 10 vials control’ under product options. This includes 20 vials of 500ug water-soluble CTZ and 10 vials of control. Please inquire if larger quantities of Cat.#3031-10+C are needed.
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| 3551 | e-Coelenterazine formulated to be water-soluble and safe for ... |
e-Coelenterazine formulated to be water-soluble and safe for in vivo imaging applications with Renilla Luciferase.
Package Contents: 500 ugs – 1 vial of 500 ugs lyophilized e-CTZ-SOL
e-Coelenterazine is often dissolved in Propyleneglycol (PG) in combination with other alcohols for in vivo imaging studies. Large intravenous doses of PG given over a short period of time can be toxic, including hyperosmolality, increased anion gap metabolic acidosis (due to lactic acidosis), acute kidney injury, and sepsis-like syndrome.
This can be avoided using water-soluble e-Coelenterazine. It is formulated to be isosmotic and easily secreted by the kidneys. Thus save for repeated i.v. injections. Water soluble e-Coelenterazine can be injected up to a 500 µg/100 µl concentration, achieving very high e-Coelenterazine levels within the body and leading to high Renilla luciferase signals. Application Example
ControlsWe offer the solubilization vehicle without the e-Coelenterazine as injection control. Any side effects due to the injection process can be easily monitored. One vial of the control (Cat. #3551-10+C) contains the same amount of solubilizer as one vial of Cat.#3551. The control will be dissolved in sterile water to create an iso-osmotic solution ready to inject. To order control: please select ’10 mgs + 10 vials control’ under product options. This includes 20 vials of 500ug water-soluble CTZ and 10 vials of control. Please inquire if larger quantities of Cat.#3551-10+C are needed.
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| 301-INJ | Injectable h-Coelenterazine, highly pure (99%) and optimized ... | 
Injectable h-Coelenterazine, highly pure (99%) and optimized for in vivo research.
Benzyl Coelenterazine (h-CTZ) in sterile injection vials with a low retention volume. Each kit is supplied with two ‘Inject-A-Lume’ vials of 500 µg h-CTZ and a sterile ‘Fuel-Inject’ diluent. For animal use only. | |||||||||||
| 3011 | Water-soluble h-Coelenterazine is formulated to be water-sol ... | 
Water-soluble h-Coelenterazine is formulated to be water-soluble and safe for in vivo imaging applications with Renilla Luciferase.
Package Contents: 500 ugs – 1 vial of 500 ugs lyophilized SOL-CTZ-h Coelenterazine compounds are often dissolved in Propyleneglycol (PG) in combination with other alcohols for in vivo imaging studies. Large intravenous doses of PG given over a short period of time can be toxic, including hyperosmolality, increased anion gap metabolic acidosis (due to lactic acidosis), acute kidney injury, and sepsis-like syndrome.
This can be avoided using water-soluble h-Coelenterazine. It is formulated to be isosmotic and easily secreted by the kidneys. Thus save for repeated i.v. injections. Water-soluble h-Coelenterazine can be injected up to a 500 µg/100 µl concentration, achieving very high Coelenterazine levels within the body and leading to high luciferase signals. h-Coelenterazine is one of the preferred substrates for Renilla Luciferase.
If you use Gaussia Luciferase in your assay please use Cat. #3031
Advantages of water-soluble Coelenterazine
ControlsWe offer the solubilization vehicle without the h-Coelenterazine as injection control. Any side effects due to the injection process can be easily monitored, especially in sensitive applications like bioluminescent driven Optogenetics. One vial of the control (Cat. #3011-10+C) contains the same amount of solubilizer as one vial of Cat.#3011. The control will be dissolved in sterile water to create an iso-osmotic solution ready to inject. To order control: please select ’10 mgs + 10 vials control’ under available sizes. This includes 20 vials of 500 µg water-soluble h-CTZ and 10 vials of control. Please inquire if larger quantities of Cat.#3011-10+C are needed. | |||||||||||
| 355-INJ | e-CTZ is now available in sterile injection vials with a low ... | 
e-CTZ is now available in sterile injection vials with a low retention volume. Each kit is suppled with two ‘Inject-A-Lume’ vials of 500 ug e-CTZ and 500 ul of steril ‘Fuel-Inject’ diluent. It was reported that this analogue enhances the signal by two-fold in Renilla Luciferase in vivo studies.
e-coelenterazine or Coelenterazine-E (e-CTZ) is a synthetic analogue of native Coelenterazine with an additional ethyl group, forming an additional ring system. First described by Shimomura in 1989. For Renilla muelleri Luciferase, an increase of 750% in initial intensity and 137% in total light was observed using e-Coelenterazine compared to naitve Coelenterazine.
Note: In will not be utilized by Gaussia Luciferase. |
Coelenterazine (native-CTZ) in sterile injection vials with a low retention volume. Each kit is supplied with two ‘Inject-A-Lume’ vials of 500 µg CTZ and 500 µl of sterile ‘Fuel-Inject’ diluent.