Luciferins & Substrates (NanoFuel)

Assay Reagents

Luciferases & GFPs

Antibodies

Vectors (NanoLights)

Keywords:

cAMP reporter • CRE/CREB • in vitro • in vivo • Kit • New • Popular

in vitro Products

Cat#Name
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Native Coelenterazine in a water-soluble form for in vitro ...
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Native Coelenterazine in a water-soluble form for in vitro applications with Gaussia Luciferase.

For additional convenience native Coelenterazine is now available in a water-soluble form aliquoted in tubes that will give you a 50 µM solution by adding 10 ml of buffer.

Content native Coelenterazine + additives for water-solubility
Packaging size adding 10 ml buffer will give a 50 µM solution, adding 5 ml buffer will give a 100 µM solution.
Application add an aequeous buffer of your choice (e.g. PBS), vortex and use for luminometer assays.

Application Example

CTZ-SOLinvitro

Advantages of water-soluble Coelenterazine

  • convenient to use, no pre-dissolving of h CTZ in alcohol necessary
  • can be used with different buffers for assay optimization 
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Gaussia(M2)-Luciferase-Streptavidin is a conjugate of one of ...
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Gaussia(M2)-Luciferase-Streptavidin is a conjugate of one of the brightest luciferases and a protein with the highest known affinity in nature. This fusion protein can be used to detect trace amounts of biotinylated protein, DNA, or other biotinylated bio-molecules.

 

Compound ID

Gaussia(M2)-Streptavidin [GLuc-SA]

Appearance clear liquid as 1 mg/ml in buffer, aliquoted as 50 µg

In experiments we were able to visualize the GLuc luminescence on the surface of Agarose-Biotin beads using a standard light microscope in total darkness. GLuc-SA was engineered by connecting one molecule of GLuc to one Streptavidin subunit. Streptavidin consists of 4 subunits with theoretically 4 biotin binding sites. Purified Streptavidin will have the ability to bind between 3.2 and 3.9 biotin molecules. Due to extensive purification steps, no free Streptavidin is present and cross-linking will be prevented, enabling quantitative read-outs in luminescent assays.

GLuc-SA_agarose_bead

  • bioluminescent detection of biotinylated biomolecules (protein, DNA, peptides) in ELISA-style assays
  • wide dynamic range
  • low background (no interfering BSA/FCS is used in this reagent)
  • highly sensitive
  • strong biotin binding
  • bioluminescent surface labeling generating high signal/noise ratios
  • overcoming problems like autofluorescence
  • recommended equipment (Olympus LV-200)
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A water-soluble form for in vitro  applications. For ad ...
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A water-soluble form for in vitro  applications.

For additional convenience h-Coelenterazine is now available in a water-soluble form aliquoted in tubes that will give you a 50 µM solution by adding 10 ml of buffer.

Content h-Coelenterazine + non-toxic additives for water-solubility
Packaging sizeadding 10ml buffer will give a 50µM solution, adding 5 ml buffer will give a 100µM solution.
ApplicationJust add an aequeous buffer of your choice (e.g. PBS), vortex and use for luminometer assays. 

Advantages of water-soluble Coelenterazine

  • convient to use, no pre-dissolving of h CTZ in alcohol necessary
  • can be used with different buffers for assay optimization 
CAT-3692a.product-thumb 3692
Methoxy e-Coelenterazine (Prolume Purple) formulated to be w ...
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Methoxy e-Coelenterazine (Prolume Purple) formulated to be water-soluble for in vitro applications with Renilla Luciferase.

Prolume Purple exhibits an up to 13-fold higher luminescence than CTZ400a (“deep blue C”).

PP-SOL-product
ContentMethoxy e-Coelenterazine + non-toxic additives for water-solublity.
Packaging Sizeadding 10ml of buffer will give a 50 μM solution, adding 5 ml buffer will give a 100 μM solution.
ApplicationJust add your buffer, vortex and use with for example in BRET assays

For details about the properties of Prolume Purple please refer to the information listed for Prolume Purple (Cat.#369) . The emission maximum with Renilla Luciferase is 405 nm thus emitting a purple light. Nanolight™ Technologies developed Prolume Purple. Patent pending.

Advantages of water-soluble Coelenterazine

  • convenient to use, no pre-dissolving of h CTZ in alcohol necessary
  • can be used with different buffers for assay optimization 
CAT-3702.product-thumb 3702
v-Coelenterazine is an analogue of Coelenterazine that cause ...
CAT-3702.product-thumb

v-Coelenterazine is an analogue of Coelenterazine that causes a shift in the emission-spectrum to longer wavelengths (515nm and above). Renilla Luciferase is the preferred catalyst for this compound. For more details see here.

Content v Coelenterazine + additives for water-solubility
Packaging Size adding 10ml buffer will give a 50µM solution, adding 5 ml buffer will give a 100µM solution.
Application Just add an aequeous buffer of your choice (e.g. PBS), invert a few times and use for luminometer assays.
Luciferase Renilla Luiferase and its mutated versions (RLuc2, RLuc8, Green Renilla Luciferase)
v-CTZ_600dpi

Advantages of water-soluble Coelenterazine

  • convenient to use, no pre-dissolving of h CTZ in alcohol necessary
  • can be used with different buffers for assay optimization 
CAT-399.product-thumb 398
Designed by NanoLight to increase the solubility of v-Coele ...
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Designed by NanoLight to increase the solubility of v-Coelenterazine and Methoxy v-Coelenterazine-Methoxy. Both compounds have a very low solubility in Ethanol and Methanol. Using NanoFuel-V solvent gives you v-CTZ concentrations of up to 4 mg/ml.

1 ml/tube

Optimized for IN VITRO use with following Coelenterazine analogs:

CAT#370 v-Coelenterazine

CAT#361 Prolume Purple V (Methoxy-v-Coelenterazine-Methoxy)

 

It is not designed for IN VIVO use.

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