A photoprotein originating from the jellyfish Aequorea victoria emits light in the presence of a trace amount of Ca2+ without the requirement of any other cofactor. Aequorin was “charged” with unmodified (native) Coelenterazine and will emit light at 465 nm upon Ca2+ contact.
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Aequorin photoprotein (untagged) is recombinantly produced in E. coli, purified via multi-step chromatography and appears as a yellow liquid.
Concentration
2 mg/ml in 1.2M (NH4)2SO4, 250 μl per tube
Sequence
MTSKQYSVKLTSDFDNPRWIGRHKHMFNFLDVNHNGKISLDEMVYKASDIVINN
LGATPEQAKRHKDAVEAFFGGAGMKYGVETDWPAYIEGWKKLATDELEKYAKNE
PTLIRIWGDALFDIVDKDQNGAITLDEWKAYTKAAGIIQSSEDCEETFRVCDIDESG
QLDVDEMTRQHLGFWYTMDPACEKLYGGAVPApplications
positive control in Ca2+ assays
Purity > 95% by SDS-PAGE
Certificates & Data
Certificate of Analysis
Safety Data Sheet
Similar Products
By Category (Photoproteins)
- 307LAequorin photoprotein (liquid)
- 307PAequorin photoprotein (lyophilized)