This cAMP reporter contains the control of multimerized cAMP response element (CRE) located upstream of the secreted Gaussia Luciferase (wt) – HRP (horseradish peroxidase) gene fusion. Expression of the GLuc-HRP fusion is induced by intracellular cAMP as a result of binding of the cAMP response element binding protein (CREB) to CRE. Secreted GLuc-HRP can be measured in the supernatant.
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- Protocols & Data
Map
Application
- Expressed Gaussia Luciferase – HRP is secreted into the supernatant, thus cell lysis is not necessary to measure activity. Ideal for multiple sampling in time-dependent assays
- Transcription regulation, virus-cell interactions, compound screening, post-translational modifications, GPCR signaling, cell signaling, promoter analysis.
- End-user preference on bioluminescence or colorimetric detection
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cAMP reporter
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