Mammalian (human) codon optimized Gaussia luciferase with N-termnal secretion signal. This product can be used as a positive control vector is very useful in evaluating the efficiency of transgene expression using Gaussia luciferase as a reporter. This vector has both Ampicillin resistance and Neomycin resistance. Therefore it can be easily propagated in E. coli and can be used to establish stable cell lines expressing Gaussia luciferase.
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Map
Sequence
202 pCMV-GLUC-1_pdf
Application
Gaussia luciferase is used as a reporter in conjunction with CRISPR to investigate the function of noncoding RNAs (ncRNA) : Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Shechner DM, Hacisuleyman E, Younger ST, Rinn JL. July 2015. PubMed
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