We offer a special discount if you purchase the lysis-buffer with one of our Luciferase Assays. Please select “add Lysis” under product options on the Luciferase buffer website.
For intracellular expressed Gaussia Luciferase please use the lysisbuffer provided with the GLuc FLASH kit (Cat.#319)
The buffer can be stored at 4°C for up to one year or at least for 5 years at -20°C.
Cypridina was first characterized by Dr Osamu and Akemi Shimomura from “Umi Hotaru” Japanese Sea Fireflies in 1966. These are bottom dwelling ostracods that emit a light stream into water when disturbed presumably to deter predation. Cypridina Luciferase was first cloned by Eric Thompson, and S. Nagata under Fred Tsuji in Japan 1989. Cypridina is a 555 amino acid, 62.171 KDa protein that oxidizes the luciferin to produce blue light at 462 nm Em Max. The advantage of using the Cypridna system: it does not cross react with Coelenterazine or Firefly d-Luciferin so it may be used in multiplex assays. It is brighter then Firefly Luciferase.
Natural occurring Cypridina Luciferin is 100% S-form. NanoLights Cypridina synthesized compound is an enantiomeric mixture of 70% S and 30% R form. The recently published paper describes that the R-form is also a luminous substrate for Cypridina Luciferase with a 3-fold lower turnover rate and not a competitive inhibitor as previously thought. Therefore, a negative impact on Cypridina bioluminescent assays is not expected.
We would like to thank the authors for the detailed analysis of our product.
Kanie S, Wu C, Kihira K, Yasuno R, Mitani Y, Ohmiya Y. Bioluminescence of (R)-Cypridina Luciferin with Cypridina Luciferase. Int J Mol Sci. 2024 Feb 26;25(5):2699. Inouye S. Pink-colored Cypridina luciferase from Cypridina (Vargula) hilgendorfii: Oxygen-triggered luminescence reaction following NAD(P)H-FMN flavin reductase reaction. Biochem Biophys Res Commun. 2025 Feb 2;747:151285.
The advantages of lyophilized luciferins are:
pre-aliquoted in small amounts, fresh substrate for every set of experiments
long shelf-life (packed under Argon)
faster to dissolve due to its fine crystal structure
consistent quality between aliquotes
The luciferin is shipped as filtered, lyophilized, batch controlled substrate
Package Contents:500 µgs – 1 vial of 500 µgs lyophilized SOL-6h-F-CTZ1 mgs – 2 vials of 500 µgs lyophilized SOL-6h-F-CTZ10 mgs – 20 vials of 500 ugs lyophilized SOL-6h-F-CTZ
6h-F-Coelenterazine is often dissolved in Propyleneglycol (PG) in combination with other alcohols for in vivo imaging studies. Large intravenous doses of PG given over a short period of time can be toxic, including hyperosmolality, increased anion gap metabolic acidosis (due to lactic acidosis), acute kidney injury, and sepsis-like syndrome.
This can be avoided using water-soluble 6h-F-Coelenterazine. It is formulated to be isosmotic and easily secreted by the kidneys. Thus save for repeated i.v. injections. Water soluble Coelenterazine can be injected up to a 500 µg/100 µl concentration, achieving very high Coelenterazine levels within the body and leading to high Gaussia luciferase signals.
Application Example
Controls
We offer the solubilization vehicle without the Coelenterazine as injection control. Any side effects due to the injection process can be easily monitored, especially in sensitive applications like bioluminescent driven Optogenetics. One vial of the control (Cat. #3481-10+C) contains the same amount of solubilizer as one vial of Cat.#3481. The control will be dissolved in sterile water to create an iso-osmotic solution ready to inject.
To order control: please select ’10 mgs + 10 vials control’ under product options. This includes 20 vials of 500 µg water-soluble 6h-F-CTZ and 10 vials of control.
Please inquire if larger quantities of Cat.#3481-10+C are needed.
