A photoprotein originating from the jellyfish Aequorea victoria emits light in the presence of a trace amount of Ca2+ without the requirement of any other cofactor. Aequorin was “charged” with unmodified (native) Coelenterazine and will emit light at 465 nm upon Ca2+ contact.
- Details & Info
- Protocols & Data
- Related Products
Aequorin photoprotein (untagged) is recombinantly produced in E. coli, purified via multi-step chromatography and appears as a white lyophilized powder.
Reconstitution
with Ca2+ free double distilled water
Sequence
MTSKQYSVKLTSDFDNPRWIGRHKHMFNFLDVNHNGKISLDEMVYKASDIVINN
LGATPEQAKRHKDAVEAFFGGAGMKYGVETDWPAYIEGWKKLATDELEKYAKNE
PTLIRIWGDALFDIVDKDQNGAITLDEWKAYTKAAGIIQSSEDCEETFRVCDIDESG
QLDVDEMTRQHLGFWYTMDPACEKLYGGAVPApplication
postitive control in Ca2+ assays
Purity > 95% by SDS-PAGE
Certificates & Data
Certificate of Analysis
Safety Data Sheet
By Category (Photoproteins)
- 307LAequorin photoprotein (liquid)
- 307PAequorin photoprotein (lyophilized)