A 2-deoxy derivative of native Coelenterazine.
Up to 20-fold higher initial light-output than native Coelenterazine with Apoaequorin.
Reduced but longer lasting kinetic with Renilla luciferase.
We recommend using CAT#399 NanoFuel Solvent for dissolving and stabilizing native Coelenterazine.
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Compound ID Coelenterazine h or 2-Deoxycoelenterazine Molecular Formula
C26H21N3O2 CAS# 50909-86-9 IUPAC Name 2,8-dibenzyl-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one MW 407.5 g/mol Package Contents:
500 ugs – 1 vial of 500 ugs lyophilized CTZ-h
1 mgs – 2 vials of 500 ugs lyophilized CTZ-h
5 mgs – 1 vial of 5 mgs lyophilized CTZ-h
10 mgs – 2 vials of 5 mgs lyophilized CTZ-hThe advantages of lyophilized luciferins are:
- Pre-aliquoted in small amounts, fresh substrate for every set of experiments
- long shelf-life (packed under Argon)
- faster to dissolve due to its fine crystal structure
- consistent quality between aliquotes
The luciferin is shipped as filtered, lyophilized, batch controlled substrate
Protocols & Manuals
Certificates & Data
Suggestion
- CAT# 3011h-Coelenterazine-SOL (in vivo)Water-soluble h-Coelenterazine is formulated to be water-soluble and safe for in vivo imaging applications with Renil...read morel
- CAT# 3012h-Coelenterazine-SOL (in vitro)A water-soluble form for in vitro applications. For additional convenience h-Coelenterazine is now available in a ...read morel
- CAT# 301-INJh-Coelenterazine-INJInjectable h-Coelenterazine, highly pure (99%) and optimized for in vivo research. read morel
- CAT# 399NanoFuel SolventNanoFuel® Solvent is NanoLight’s solution to the problem associated with the solubility of Coelenterazine and the man...read morel
By Category (Coelenterazines)
- 303Coelenterazine native
- 301Coelenterazine h
- 340Coelenterazine 400a
- 355e-Coelenterazine
- 345Coelenterazine F
- 346e-Coelenterazine-F
- 370v-Coelenterazine
- 397NanoFuel-400a Solvent
- 398NanoFuel-V Solvent
- 399NanoFuel Solvent
For maximum stability and shelf-life of the substrate we recommend NanoFuel Solvent (Cat.# 399).
As an alternative you may use Methanol to dissolve h-CTZ.
Related Products
Coelenterazine h
The photoprotein Aequorin is showing an up to 20-fold higher inital intensity with h-CTZ compared to native Coelenterazine.
h-CTZ is also the preferred substrate for Renilla Luciferase in HTS (high throughput screens) due to its prolonged glow kinetic.
Related Products
Coelenterazine h
Suggested Articles
PMID 2803250 (view abstract) Author Kishi Y Date 1989 Aug 1 Journal The Biochemical journal PMID 9144537 (view abstract) Author Shimomura O Date 1997 Apr 17 Journal Biochemical and biophysical research communications PMID 8458875 (view abstract) Author Trewavas AJ Date 1993 Apr Journal The Journal of cell biology