Luciferins & Substrates (NanoFuel)

Assay Reagents

Luciferases & GFPs

Antibodies

Vectors (NanoLights)

Assay Reagents Products

Cat#Name
CAT#320(b).product-thumb featured 320
This kit includes a buffer that will prolong your Gaussia si ...
CAT#320(b).product-thumb
Introducing a new one-step GLOW Assay kit for high-throughput screenings (HTS). This kit includes a buffer that will prolong your Gaussia signal over several hours. The half-life of Gaussia in this buffer system is between 2 to 3 hours.  

Advantages

  • one-step assay reagent (lysis-, dilution- and assay-buffer in one solution)
  • stable luminescent signal (2-3 hours half-life)
  • detection of secreted Gaussia Luciferase
  • detection of intracellular expressed Gaussia Luciferase
  • low background, thus high Signal to Noise ratio

320-kinetic

CAT-319b.product-thumb 319
NanoFuel® FLASH Assay for Gaussia Luciferase The NanoFue ...
CAT-319b.product-thumb
NanoFuel® FLASH Assay for Gaussia Luciferase The NanoFuel® FLASH Assay kit provides enough buffer for approx. 1000 assays and performs best with a luminometer equipped with an injection port. For assays requiring a longer lasting luminescence (e.g. high-throughput screenings) or if sensitivity is not an issue we recommend our NanoFuel GLOW Assay kit (Cat. #320).  

Advantages of the FLASH Assay Reagent for Gaussia Luciferase:

  • creates optimal conditions for GLuc and mutated GLuc versions
  • low background, thus higher S/N ratio
  • higher overall signal
  • buffer has protein stabilizing properties

319-comparison

CAT-324b.product-thumb 324
NanoFuel® FLASH Assay for Oplophorus Luciferases NanoFuel ...
CAT-324b.product-thumb

NanoFuel® FLASH Assay for Oplophorus Luciferases

NanoFuel FLASH assay buffer is an alternative for assays that are using substrates like native Coelenterazine or Coeleterazine analogs to achieve even higher bioluminescent readouts with Oplophorus luciferases like NanoLuc® or NanoKAZ.

Kit content

  • 50 ml NanoFuel® FLASH Reagent for Oplophorus Luciferase
  • 1 ml 50X NLuc FLASH Substrate

Characteristics

  • one-step assay
  • bright luminescent signal for at least a few minutes
  • higher sensitivity than CTZ or Furimazine
  • low background, higher S/N ratio
  • enough reagent for 1000 assays
  • luminometer with injection port is recommended
  • although the substrate will diffuse into the cells this process can be accelerated by a lysis step, please select “add Lysis” under product options to add 50ml of 5x Universal Lysisbuffer (CAT# 333) to your order

Graph below: HEK293 cells were transformed with NLuc and assayed 2 days later using a luminometer with injection port. Compared to other NLuc assay buffers, Cat. #324 increased the inital lightoutput with a reduced signal half-life. This data was kindly provided by the Neuroscience Department at Central Michigan University.

324_comparison_graph

CAT-325c.product-thumb 325
NanoFuel® GLOW Assay for Oplophorus Luciferases Introdu ...
CAT-325c.product-thumb
NanoFuel® GLOW Assay for Oplophorus Luciferases Introducing a new GLOW Assay kit that will work with Oplophorus Luciferase and its variants NanoKAZ. 

Kit content

  • 50 ml NLuc GLOW buffer for Oplophorus Luciferases
  • 1 ml 50x Substrate for Oplophorus Luciferases

Advantages

  • one-step assay
  • stable luminescent signal (half-life approx. 1.5 hrs)
  • affordable
  • high sensitivity
  • low background, higher S/N ratio
  • enough reagent for 1000 assays
  • no injection port needed on the luminometer

 

Oplo-kinetic

 

For a more intense FLASH-type kinetic please see our NLuc FLASH Assay Cat. #324

CAT-318b.product-thumb 318
The NanoFuel® Assay kit for Firefly Luciferase kit provides ...
CAT-318b.product-thumb
The NanoFuel® Assay kit for Firefly Luciferase kit provides enough buffer for approx. 1000 assays. Optimized buffer conditions will give you the best signal over a half-life of 45 min. Therefore no injection luminometer is required. This kit can be used for high-throughput screenings due to its steady signal.

FLuc Assay Kit content

  • 50 ml       FLuc Buffer – provides optimal reaction conditions
  • 1 vial        Luciferin substrate (please add to buffer before use) 

Properties of FLuc Assay Buffer

  • creates optimal conditions for FLuc
  • low background, thus higher S/N ratio
  • very high and steady signal
  • can be used in high-throughput experiments
  • NOTE: this buffer does not contain any detergent,
    intracellular expressed FLuc will require lysis
  • for intracellular assays a 5x Universal Lysis Buffer (Cat.#333) can be purchased, please select “add lysis” under product options

318_comp_chart

nl-product-placeholder 333
A buffer used for cells expressing Firefly, Renilla and Oplo ...
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This buffer provides optimal lysis conditions for cells expressing Firefly, Renilla and Oplophorus (NanoLuc, NanoKAZ) luciferases.
The proprietary formula has stabilizing properties and will enhance the performance of the expressed luciferase.

This lysis-buffer is compatible with the following Luciferase Assay Reagents

Cat. #318 Firefly Assay Reagent
Cat. #324 NLuc FLASH buffer

We offer a special discount if you purchase the lysis-buffer with one of our Luciferase Assays. Please select “add Lysis” under product options on the Luciferase buffer website.

For intracellular expressed Gaussia Luciferase please use the lysisbuffer provided with the GLuc FLASH kit (Cat.#319)

The buffer can be stored at 4°C for up to one year or at least for 5 years at -20°C.

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