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NanoKaz (NLuc) Assay Products

	Cat#	Name
	324	NLuc FLASH Assay NanoFuel® FLASH Assay for Oplophorus Luciferases NanoFuel ...		NanoFuel® FLASH Assay for Oplophorus Luciferases NanoFuel FLASH assay buffer is an alternative for assays that are using substrates like native Coelenterazine or Coeleterazine analogs to achieve even higher bioluminescent readouts with Oplophorus luciferases like NanoLuc® or NanoKAZ. Kit content 50 ml NanoFuel® FLASH Reagent for Oplophorus Luciferase 1 ml 50X NLuc FLASH Substrate Characteristics one-step assay bright luminescent signal for at least a few minutes higher sensitivity than CTZ or Furimazine low background, higher S/N ratio enough reagent for 1000 assays luminometer with injection port is recommended although the substrate will diffuse into the cells this process can be accelerated by a lysis step, please select “add Lysis” under product options to add 50ml of 5x Universal Lysisbuffer (CAT# 333) to your order Graph below: HEK293 cells were transformed with NLuc and assayed 2 days later using a luminometer with injection port. Compared to other NLuc assay buffers, Cat. #324 increased the inital lightoutput with a reduced signal half-life. This data was kindly provided by the Neuroscience Department at Central Michigan University.
	325	NLuc GLOW Assay NanoFuel® GLOW Assay for Oplophorus Luciferases Introdu ...		NanoFuel^® GLOW Assay for Oplophorus Luciferases Introducing a new GLOW Assay kit that will work with Oplophorus Luciferase and its variants NanoKAZ. Kit content 50 ml NLuc GLOW buffer for Oplophorus Luciferases 1 ml 50x Substrate for Oplophorus Luciferases Advantages one-step assay stable luminescent signal (half-life approx. 1.5 hrs) affordable high sensitivity low background, higher S/N ratio enough reagent for 1000 assays no injection port needed on the luminometer For a more intense FLASH-type kinetic please see our NLuc FLASH Assay Cat. #324