Luciferins & Substrates (NanoFuel) Products
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| 309 | Coenzyme A will prolong the signal of Firefly Luciferase Bio ... | 
Coenzyme A will prolong the signal of Firefly Luciferase Bioluminescence 
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| 305 | Known also as Vargula Luciferin or Vargulin
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Known also as Vargula Luciferin or Vargulin
Cypridina was first characterized by Dr Osamu and Akemi Shimomura from “Umi Hotaru” Japanese Sea Fireflies in 1966. These are bottom dwelling ostracods that emit a light stream into water when disturbed presumably to deter predation. Cypridina Luciferase was first cloned by Eric Thompson, and S. Nagata under Fred Tsuji in Japan 1989. Cypridina is a 555 amino acid, 62.171 KDa protein that oxidizes the luciferin to produce blue light at 462 nm Em Max. The advantage of using the Cypridna system: it does not cross react with Coelenterazine or Firefly d-Luciferin so it may be used in multiplex assays, brighter then Firefly Luciferase The advantages of lyophilized luciferins are:
The luciferin is shipped as filtered, lyophilized, batch controlled substrate | |||||||||||||
| 306 | Firefly D-Luciferin Free Acid >99% (Photinus pyralis, pyr ... | 
Firefly D-Luciferin Free Acid >99% (Photinus pyralis, pyrophorus, and Elateridea substrate luciferins) This is the FREE ACID and not a water soluble salt, such as the Potassium or Sodium salts. This requires reconstitution using alkaline buffers. 
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| 369 | Methoxy e-Coelenterazine analog quenching the emission maxi ... | 
Prolume Purple or methoxy e-Coelenterazine (Me-O-e-CTZ) is a new synthetic analogue of e-Coelenterazine with an additional methoxy group quenching the emission maximum from 475 nm to 405 nm. Nanolight™ Technologies developed Prolume Purple. (US Patent 2013/057660) Prolume Purple exhibits an up to 13-fold higher luminescence than CTZ400a (“deep blue C”). 
Comparison to CTZ400a
Emission spec with Renilla Luciferases
The advantages of lyophilized luciferins are:
The luciferin is shipped as filtered, lyophilized, batch controlled substrate | |||||||||||||
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| 367 | Prolume Purple II or Methoxy-Coelenterazine-Methoxy (Me-O- ... |
Prolume Purple II or Methoxy-Coelenterazine-Methoxy (Me-O-CTZ-O-Me) is a new synthetic analogue of Coelenterazine with two additional methoxy groups emitting at 400nm with Renilla Luciferase 8 (RLuc8) and Green Renilla Luciferase. Nanolight™ Technologies developed Prolume Purple II (US Patent 2013/057660).
Prolume Purple II exhibits an up to 5-fold higher luminescence than CTZ400a (“deep blue C”).
Activity compared to CTZ-400a
Emission specs with RLuc8
The advantages of lyophilized luciferins are:
The luciferin is shipped as filtered, lyophilized, batch controlled substrate | |||||||||||||
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| 365 | Methoxy-eCoelenterazine-F (Me-O-eCTZ-F) is a new synthetic a ... |
Methoxy-eCoelenterazine-F (Me-O-eCTZ-F) is a new synthetic analogue of e-Coelenterazine-F with an additional methoxy groups emitting at 410nm with Green Renilla Luciferase. Nanolight™ Technologies developed Prolume Purple III. (US Patent 2013/057660) Prolume Purple III exhibits an up to 10-fold higher luminescence than CTZ400a (“deep blue C”).
If you are using RLuc8 or wildtyp Renilla Luc please use Prolume Purple (Cat.# 369). 
Activity compared to other CTZs
Emission specs with Green RLuc
The advantages of lyophilized luciferins are
The luciferin is shipped as filtered, lyophilized, batch controlled substrate for 250µg and 1mg (4x 250µg) aliquots.
10 mgs are packed as regular powder in one tube.
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| 363 | Prolume Purple IV or Methoxy-Coelenterazine-Iodine (Me-O-CT ... |
Prolume Purple IV or Methoxy-Coelenterazine-Iodine (Me-O-CTZ-I) is a new synthetic analogue of Coelenterazine-I with an additional methoxy group. So far we were not able to determine a luciferase that is working with this unique compound. Activity with RLuc8, Rluc3 and Green Renilla Luciferase were very low.
Nanolight™ Technologies developed Prolume Purple IV. (US Patent 2013/057660).
Advantages
The luciferin is shipped as filtered, lyophilized, batch controlled substrate for 250µg and 1mg (4x 250µg) aliquots.
10 mgs are packed as regular powder in one tube.
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| 360 | Nanolight™ Technologies patented methoxy Coelenterazine c ... | 
Nanolight™ Technologies patented methoxy Coelenterazine compounds are now available as a comprehensive sampler kit. Nanolight Technology is pleased to introduce these newly developed coelenterazine compounds. Your feedback is appreciated, if you find any additional luciferases or luciferase mutants that work with these compounds, please contact us at info@prolume.com. Prolume Purple Sampler content (250 ug each) | |||||||||||||
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| 361 | Methoxy-v-Coelenterazine-Methoxy (Me-O-v-CTZ-O-Me) is a new ... |
Methoxy-v-Coelenterazine-Methoxy (Me-O-v-CTZ-O-Me) is a new synthetic analogue of v-Coelenterazine with two additional methoxy groups. So far we were not able to determine a luciferase that is working with this unique compound. Activities with RLuc8, Rluc3 and Green Renilla Luciferase are very low. 
Advantages
The luciferin is shipped as filtered, lyophilized, batch controlled substrate for 250µg and 1mg (4x 250µg) aliquots.
10 mgs are packed as regular powder in one tube.
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| 303 | >95% pure, native Coelenterazine. lyophilized. | 
Coelenterazine Free Base purity >95% The most abundant form of Coelenterazine found in nature.
We recommend using CAT#399 NanoFuel Solvent for dissolving and stabilizing native Coelenterazine. 
Package Contents: 500 ugs – 1 vial of 500 ugs lyophilized CTZ The advantages of lyophilized luciferins are:
This luciferin is shipped as filtered, lyophilized, batch controlled substrate.
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| 303-INJ | Injectable native Coelenterazine, highly pure (99.7%) and op ... | 
Injectable native Coelenterazine, highly pure (99.7%) and optimized for in vivo research.
Recommended path of injection: intra-peritoneal cavity. For animal use only. | |||||||||||||
| 3032 | Native Coelenterazine in a water-soluble form for in vitro ... | 
Native Coelenterazine in a water-soluble form for in vitro applications with Gaussia Luciferase. For additional convenience native Coelenterazine is now available in a water-soluble form aliquoted in tubes that will give you a 50 µM solution by adding 10 ml of buffer.
Application Example
Advantages of water-soluble Coelenterazine
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| 3031 | Native Coelenterazine formulated to be water-soluble and saf ... | 
Native Coelenterazine formulated to be water-soluble and safe for in vivo imaging applications with Gaussia Luciferase.
Package Contents: 500 ugs – 1 vial of 500 ugs lyophilized SOL-CTZ
Coelenterazine is often dissolved in Propyleneglycol (PG) in combination with other alcohols for in vivo imaging studies. Large intravenous doses of PG given over a short period of time can be toxic, including hyperosmolality, increased anion gap metabolic acidosis (due to lactic acidosis), acute kidney injury, and sepsis-like syndrome.
This can be avoided using water-soluble Coelenterazine. It is formulated to be isosmotic and easily secreted by the kidneys. Thus save for repeated i.v. injections. Water soluble Coelenterazine can be injected up to a 500 µg/100 µl concentration, achieving very high Coelenterazine levels within the body and leading to high Gaussia luciferase signals. Application Example
ControlsWe offer the solubilization vehicle without the Coelenterazine as injection control. Any side effects due to the injection process can be easily monitored, especially in sensitive applications like bioluminescent driven Optogenetics. One vial of the control (Cat. #3031-10+C) contains the same amount of solubilizer as one vial of Cat.#3031. The control will be dissolved in sterile water to create an iso-osmotic solution ready to inject. To order control: please select ’10 mgs + 10 vials control’ under product options. This includes 20 vials of 500ug water-soluble CTZ and 10 vials of control. Please inquire if larger quantities of Cat.#3031-10+C are needed.
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| 301 | A 2-deoxy derivative of native Coelenterazine.
Up to 20-f ... | 
A 2-deoxy derivative of native Coelenterazine. Up to 20-fold higher initial light-output than native Coelenterazine with Apoaequorin.
We recommend using CAT#399 NanoFuel Solvent for dissolving and stabilizing native Coelenterazine. 
Package Contents: 500 ugs – 1 vial of 500 ugs lyophilized CTZ-h
The advantages of lyophilized luciferins are:
The luciferin is shipped as filtered, lyophilized, batch controlled substrate | |||||||||||||
| 301-INJ | Injectable h-Coelenterazine, highly pure (99%) and optimized ... | 
Injectable h-Coelenterazine, highly pure (99%) and optimized for in vivo research.
Benzyl Coelenterazine (h-CTZ) in sterile injection vials with a low retention volume. Each kit is supplied with two ‘Inject-A-Lume’ vials of 500 µg h-CTZ and a sterile ‘Fuel-Inject’ diluent. For animal use only. | |||||||||||||
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| 3012 | A water-soluble form for in vitro applications.
For ad ... |
A water-soluble form for in vitro applications. For additional convenience h-Coelenterazine is now available in a water-soluble form aliquoted in tubes that will give you a 50 µM solution by adding 10 ml of buffer.
Advantages of water-soluble Coelenterazine
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Coelenterazine (native-CTZ) in sterile injection vials with a low retention volume. Each kit is supplied with two ‘Inject-A-Lume’ vials of 500 µg CTZ and 500 µl of sterile ‘Fuel-Inject’ diluent.