Luciferins & Substrates (NanoFuel)

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Vectors (NanoLights)

Water-soluble CTZs Products

Cat#Name
CAT-3032.product-thumb 3032
Native Coelenterazine in a water-soluble form for in vitro ...
CAT-3032.product-thumb

Native Coelenterazine in a water-soluble form for in vitro applications with Gaussia Luciferase.

For additional convenience native Coelenterazine is now available in a water-soluble form aliquoted in tubes that will give you a 50 µM solution by adding 10 ml of buffer.

Content native Coelenterazine + additives for water-solubility
Packaging size adding 10 ml buffer will give a 50 µM solution, adding 5 ml buffer will give a 100 µM solution.
Application add an aequeous buffer of your choice (e.g. PBS), vortex and use for luminometer assays.

Application Example

CTZ-SOLinvitro

Advantages of water-soluble Coelenterazine

  • convenient to use, no pre-dissolving of h CTZ in alcohol necessary
  • can be used with different buffers for assay optimization 
CAT-3031b.product-thumb featured 3031
Native Coelenterazine formulated to be water-soluble and saf ...
CAT-3031b.product-thumb
Native Coelenterazine formulated to be water-soluble and safe for in vivo imaging applications with Gaussia Luciferase.
Contentnative Coelenterazine + non-toxic additives for water-solubility
Packaging size500 ug CTZ / vial in a freeze-dried form
Applicationjust add sterile water, vortex and use up to 500 ug CTZ in a single injection

Package Contents:

500 ugs  – 1 vial of 500 ugs lyophilized SOL-CTZ
1 mgs – 2 vials of 500 ugs lyophilized SOL-CTZ
10 mgs – 20 vials of 500 ugs lyophilized SOL-CTZ

 

Coelenterazine is often dissolved in Propyleneglycol (PG) in combination with other alcohols for in vivo imaging studies. Large intravenous doses of PG given over a short period of time can be toxic, including  hyperosmolality, increased anion gap metabolic acidosis (due to lactic acidosis), acute kidney injury, and sepsis-like syndrome.

This can be avoided using water-soluble Coelenterazine. It is formulated to be isosmotic and easily secreted by the kidneys. Thus save for repeated i.v. injections. Water soluble Coelenterazine can be injected up to a 500 µg/100 µl concentration, achieving very high Coelenterazine levels within the body and leading to high Gaussia luciferase signals.

Application Example

picture for S-CTZ webpage

Controls

We offer the solubilization vehicle without the Coelenterazine as injection control. Any side effects due to the injection process can be easily monitored, especially in sensitive applications like bioluminescent driven Optogenetics. One vial of the control (Cat. #3031-10+C) contains the same amount of solubilizer as one vial of Cat.#3031. The control will be dissolved in sterile water to create an iso-osmotic solution ready to inject.

To order control: please select ’10 mgs + 10 vials control’ under product options. This includes 20 vials of 500ug water-soluble CTZ and 10 vials of control.

Please inquire if larger quantities of Cat.#3031-10+C are needed.

 

CAT-3031b.product-thumb featured 3551
e-Coelenterazine formulated to be water-soluble and safe for ...
CAT-3031b.product-thumb
e-Coelenterazine formulated to be water-soluble and safe for in vivo imaging applications with Renilla Luciferase.
Contente-Coelenterazine + non-toxic additives for water-solubility
Packaging size500 ug e-CTZ / vial in a freeze-dried form
Applicationjust add sterile water, vortex and use up to 500 ug e-CTZ in a single injection

Package Contents:

500 ugs  – 1 vial of 500 ugs lyophilized e-CTZ-SOL
1 mgs – 2 vials of 500 ugs lyophilized e-CTZ-SOL
10 mgs – 20 vials of 500 ugs lyophilized e-CTZ-SOL

 

e-Coelenterazine is often dissolved in Propyleneglycol (PG) in combination with other alcohols for in vivo imaging studies. Large intravenous doses of PG given over a short period of time can be toxic, including  hyperosmolality, increased anion gap metabolic acidosis (due to lactic acidosis), acute kidney injury, and sepsis-like syndrome.

This can be avoided using water-soluble e-Coelenterazine. It is formulated to be isosmotic and easily secreted by the kidneys. Thus save for repeated i.v. injections. Water soluble e-Coelenterazine can be injected up to a 500 µg/100 µl concentration, achieving very high e-Coelenterazine levels within the body and leading to high Renilla luciferase signals.

Application Example

picture for S-CTZ webpage

Controls

We offer the solubilization vehicle without the e-Coelenterazine as injection control. Any side effects due to the injection process can be easily monitored. One vial of the control (Cat. #3551-10+C) contains the same amount of solubilizer as one vial of Cat.#3551. The control will be dissolved in sterile water to create an iso-osmotic solution ready to inject.

To order control: please select ’10 mgs + 10 vials control’ under product options. This includes 20 vials of 500ug water-soluble CTZ and 10 vials of control.

Please inquire if larger quantities of Cat.#3551-10+C are needed.

 

nl-product-placeholder 3012
A water-soluble form for in vitro  applications. For ad ...
nl-product-placeholder

A water-soluble form for in vitro  applications.

For additional convenience h-Coelenterazine is now available in a water-soluble form aliquoted in tubes that will give you a 50 µM solution by adding 10 ml of buffer.

Content h-Coelenterazine + non-toxic additives for water-solubility
Packaging sizeadding 10ml buffer will give a 50µM solution, adding 5 ml buffer will give a 100µM solution.
ApplicationJust add an aequeous buffer of your choice (e.g. PBS), vortex and use for luminometer assays. 

Advantages of water-soluble Coelenterazine

  • convient to use, no pre-dissolving of h CTZ in alcohol necessary
  • can be used with different buffers for assay optimization 
CAT-3011b.product-thumb 3011
Water-soluble h-Coelenterazine is formulated to be water-sol ...
CAT-3011b.product-thumb
Water-soluble h-Coelenterazine is formulated to be water-soluble and safe for in vivo imaging applications with Renilla Luciferase.
 
Content h-Coelenterazine + non-toxic additives for water-solubility
Packaging size 500 µg h-CTZ / vial in a freeze-dried form
Application just add sterile water, vortex and use up to 500 µg CTZ in a single injection

Package Contents:

500 ugs  – 1 vial of 500 ugs lyophilized SOL-CTZ-h
1 mgs – 2 vials of 500 ugs lyophilized SOL-CTZ-h
10 mgs – 20 vials of 500 ugs lyophilized SOL-CTZ-h

Coelenterazine compounds are often dissolved in Propyleneglycol (PG) in combination with other alcohols for in vivo imaging studies. Large intravenous doses of PG given over a short period of time can be toxic, including  hyperosmolality, increased anion gap metabolic acidosis (due to lactic acidosis), acute kidney injury, and sepsis-like syndrome.

This can be avoided using water-soluble h-Coelenterazine. It is formulated to be isosmotic and easily secreted by the kidneys. Thus save for repeated i.v. injections. Water-soluble h-Coelenterazine can be injected up to a 500 µg/100 µl concentration, achieving very high Coelenterazine levels within the body and leading to high luciferase signals.
 
h-Coelenterazine is one of the preferred substrates for Renilla Luciferase.
If you use Gaussia Luciferase in your assay please use Cat. #3031

Advantages of water-soluble Coelenterazine

  • non-toxic, iso-osmotic, Propylene-Glycol free, Alcohol free
  • long shelf-life, sealed under vacuum
  • dissolves fast in sterile water
  • low viscosity – ideal for injections
  • consistent quality due to small aliquot sizes

Controls

We offer the solubilization vehicle without the h-Coelenterazine as injection control. Any side effects due to the injection process can be easily monitored, especially in sensitive applications like bioluminescent driven Optogenetics. One vial of the control (Cat. #3011-10+C) contains the same amount of solubilizer as one vial of Cat.#3011. The control will be dissolved in sterile water to create an iso-osmotic solution ready to inject.

To order control: please select ’10 mgs + 10 vials control’ under available sizes. This includes 20 vials of 500 µg water-soluble h-CTZ  and 10 vials of control.

Please inquire if larger quantities of Cat.#3011-10+C are needed.

CAT-3692a.product-thumb 3692
Methoxy e-Coelenterazine (Prolume Purple) formulated to be w ...
CAT-3692a.product-thumb

Methoxy e-Coelenterazine (Prolume Purple) formulated to be water-soluble for in vitro applications with Renilla Luciferase.

Prolume Purple exhibits an up to 13-fold higher luminescence than CTZ400a (“deep blue C”).

PP-SOL-product
ContentMethoxy e-Coelenterazine + non-toxic additives for water-solublity.
Packaging Sizeadding 10ml of buffer will give a 50 μM solution, adding 5 ml buffer will give a 100 μM solution.
ApplicationJust add your buffer, vortex and use with for example in BRET assays

For details about the properties of Prolume Purple please refer to the information listed for Prolume Purple (Cat.#369) . The emission maximum with Renilla Luciferase is 405 nm thus emitting a purple light. Nanolight™ Technologies developed Prolume Purple. Patent pending.

Advantages of water-soluble Coelenterazine

  • convenient to use, no pre-dissolving of h CTZ in alcohol necessary
  • can be used with different buffers for assay optimization 
CAT-3702.product-thumb 3702
v-Coelenterazine is an analogue of Coelenterazine that cause ...
CAT-3702.product-thumb

v-Coelenterazine is an analogue of Coelenterazine that causes a shift in the emission-spectrum to longer wavelengths (515nm and above). Renilla Luciferase is the preferred catalyst for this compound. For more details see here.

Content v Coelenterazine + additives for water-solubility
Packaging Size adding 10ml buffer will give a 50µM solution, adding 5 ml buffer will give a 100µM solution.
Application Just add an aequeous buffer of your choice (e.g. PBS), invert a few times and use for luminometer assays.
Luciferase Renilla Luiferase and its mutated versions (RLuc2, RLuc8, Green Renilla Luciferase)
v-CTZ_600dpi

Advantages of water-soluble Coelenterazine

  • convenient to use, no pre-dissolving of h CTZ in alcohol necessary
  • can be used with different buffers for assay optimizationÂ