Benzyl Coelenterazine (h-CTZ) in sterile injection vials with a low retention volume. Each kit is supplied with two ‘Inject-A-Lume’ vials of 500 µg h-CTZ and a sterile ‘Fuel-Inject’ diluent.
For animal use only.
Package Contents:
500 ugs – 1 vial of 500 ugs lyophilized SOL-CTZ-h
1 mgs – 2 vials of 500 ugs lyophilized SOL-CTZ-h
10 mgs – 20 vials of 500 ugs lyophilized SOL-CTZ-h
Coelenterazine compounds are often dissolved in Propyleneglycol (PG) in combination with other alcohols for in vivo imaging studies. Large intravenous doses of PG given over a short period of time can be toxic, including hyperosmolality, increased anion gap metabolic acidosis (due to lactic acidosis), acute kidney injury, and sepsis-like syndrome.
This can be avoided using water-soluble h-Coelenterazine. It is formulated to be isosmotic and easily secreted by the kidneys. Thus save for repeated i.v. injections. Water-soluble h-Coelenterazine can be injected up to a 500 µg/100 µl concentration, achieving very high Coelenterazine levels within the body and leading to high luciferase signals.
h-Coelenterazine is one of the preferred substrates for Renilla Luciferase.
If you use Gaussia Luciferase in your assay please use Cat. #3031
Advantages of water-soluble Coelenterazine
- non-toxic, iso-osmotic, Propylene-Glycol free, Alcohol free
- long shelf-life, sealed under vacuum
- dissolves fast in sterile water
- low viscosity – ideal for injections
- consistent quality due to small aliquot sizes
Controls
We offer the solubilization vehicle without the h-Coelenterazine as injection control. Any side effects due to the injection process can be easily monitored, especially in sensitive applications like bioluminescent driven Optogenetics. One vial of the control (
Cat. #3011-10+C) contains the same amount of solubilizer as one vial of Cat.#3011. The control will be dissolved in sterile water to create an iso-osmotic solution ready to inject.
To order control: please select ’10 mgs + 10 vials control’ under available sizes. This includes 20 vials of 500 µg water-soluble h-CTZ and 10 vials of control.
Please inquire if larger quantities of Cat.#3011-10+C are needed.
Package Contents:
500 ugs – 1 vial of 500 ugs lyophilized CTZ 400a
1 mgs – 2 vials of 500 ugs lyophilized CTZ 400a
5 mgs – 1 vial of 5 mgs lyophilized CTZ 400a
10 mgs – 2 vials of 5 mgs lyophilized CTZ 400a
Advantages
- pre-aliquoted in small amounts, fresh substrate for every set of experiments
- long shelf-life (packed under Argon)
- faster to dissolve due to its fine crystal structure
- consistent quality between aliquotes
The luciferin is shipped as filtered, lyophilized, batch controlled substrate
e-coelenterazine or Coelenterazine-E (e-CTZ) is a synthetic analogue of native Coelenterazine with an additional ethyl group, forming an additional ring system. First described by Shimomura in 1989.
For Renilla muelleri Luciferase, an increase of 750% in initial intensity and 137% in total light was observed using e-Coelenterazine compared to naitve Coelenterazine.
Note: In will not be utilized by Gaussia Luciferase.
