v-Coelenterazine

Shift in emission with RLuc 8

Renilla Luciferases emission spectra

The advantages of lyophilized luciferins

pre-aliquoted in small amounts, fresh substrate for every set of experiments
long shelf-life (packed under Argon)
faster to dissolve due to its fine crystal structure
consistent quality between aliquotes

The luciferin is shipped as filtered, lyophilized, batch controlled substrate in 500 µg and 1 mg aliquots (1 mg = 2x 500 µg). 10 mgs is packed as regular powder in one tube.

pART7-Pt-GFP

Map	Sequence	Usage References
	120 Plasmid_pART7_PtGFP sequence_map PDF	120 Plasmid_pART7_PtGFP info PDF

pGLuc-KDEL-Basic

Map	Sequence
	207 pGLUC-KDEL-Basic-1 sequence_map

Application

Gaussia luciferase is used as a reporter in conjunction with CRISPR to investigate the function of noncoding RNAs (ncRNA) : Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Shechner DM, Hacisuleyman E, Younger ST, Rinn JL. July 2015. PubMed

CRE/CREB GLuc-HRP cAMP Reporter

Map

Application

Expressed Gaussia Luciferase – HRP is secreted into the supernatant, thus cell lysis is not necessary to measure activity. Ideal for multiple sampling in time-dependent assays
Transcription regulation, virus-cell interactions, compound screening, post-translational modifications, GPCR signaling, cell signaling, promoter analysis.
End-user preference on bioluminescence or colorimetric detection

Before this product can be shipped please read, sign and email the following MTA agreement to info@intouchbio.com

NanoFuel-V Solvent

1 ml/tube Optimized for IN VITRO use with following Coelenterazine analogs: CAT#370 v-Coelenterazine CAT#361 Prolume Purple V (Methoxy-v-Coelenterazine-Methoxy) It is not designed for IN VIVO use.