Prolume Purple- SOL (in vitro)

For details about the properties of Prolume Purple please refer to the information listed for Prolume Purple (Cat.#369) . The emission maximum with Renilla Luciferase is 405 nm thus emitting a purple light. Nanolight™ Technologies developed Prolume Purple. Patent pending.

Advantages of water-soluble Coelenterazine

convenient to use, no pre-dissolving of h CTZ in alcohol necessary
can be used with different buffers for assay optimization

pUC18 Rr. GFP

Sequence

e-Coelenterazine

We recommend using CAT#399 NanoFuel Solvent for dissolving and stabilizing native Coelenterazine. The luciferin is shipped as filtered, lyophilized, batch controlled substrate in 500 µg and 1 mg aliquots (1 mg = 2x 500 µg). 10 mgs is packed as regular powder in one tube.

Advantages

pre-aliquoted in small amounts, fresh substrate for every set of experiments
long shelf-life (packed under Argon)
faster to dissolve due to its fine crystal structure
consistent quality between aliquotes

pGluc-basic

Map	Sequence
	203 pGLuc-basic -1 sequence_map

Application

Gaussia luciferase is used as a reporter in conjunction with CRISPR to investigate the function of noncoding RNAs (ncRNA) : Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Shechner DM, Hacisuleyman E, Younger ST, Rinn JL. July 2015. PubMed

pUC18 h- R.M. GFP

Sequence

Renilla Mulleri Luciferase

Amino Acid sequence:

MTSKVYDPELRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENAVIFLHGNAASSYLWRHVVPHVEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTEWFKHLNLPKKIIFVGHDWGACLAFHYCYEHQDRIKAVVHAESVVDVIESWDEWPDIEEDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREIPLVKGGKPDVVEIVRNYNAYLRASHDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVKGLHFSQEDAPDEMGNYIKSFVERVLKNEQ

v-Coelenterazine-SOL (in vitro)

Advantages of water-soluble Coelenterazine

convenient to use, no pre-dissolving of h CTZ in alcohol necessary
can be used with different buffers for assay optimization

Coelenterazine F

Package Contents: 500 ugs – 1 vial of 500 ugs lyophilized CTZ-f 1 mgs – 2 vials of 500 ugs lyophilized CTZ-f 5 mgs – 1 vial of 5 mgs lyophilized CTZ-f 10 mgs – 2 vials of 5 mgs lyophilized CTZ-f

Advantages

pre-aliquoted in small amounts, fresh substrate for every set of experiments
long shelf-life (packed under Argon)
faster to dissolve due to its fine crystal structure
consistent quality between aliquotes

The luciferin is shipped as filtered, lyophilized, batch controlled substrate

pUC18 h- Pt. GFP

Sequence

pUC19 R.M. Luc

Sequence

e-Coelenterazine-F

Emission spectrum of eCTZ-F

eCTZ-F is showing a unique emission spectrum in combination with Renilla Luciferase (RLuc), emitting a high energy wide spectrum from 400 to 550 nm. Please see the manual for details. The luciferin is shipped as filtered, lyophilized, batch controlled substrate in 500 µg and 1 mg aliquots (1 mg = 2x 500 µg). 10 mgs is packed as regular powder in one tube.