Luciferins & Substrates (NanoFuel)

Assay Reagents

Luciferases & GFPs

Antibodies

Vectors (NanoLights)

Prolume Purple- SOL (in vitro)

For details about the properties of Prolume Purple please refer to the information listed for Prolume Purple (Cat.#369) . The emission maximum with Renilla Luciferase is 405 nm thus emitting a purple light. Nanolight™ Technologies developed Prolume Purple. Patent pending.

Advantages of water-soluble Coelenterazine

  • convenient to use, no pre-dissolving of h CTZ in alcohol necessary
  • can be used with different buffers for assay optimization 

pUC18 Rr. GFP

Sequence

103 pUC18-Rr-GFP sequencepdf-icon 103 pUC18-Rr-GFP sequenceview-icon

e-Coelenterazine

We recommend using CAT#399 NanoFuel Solvent for dissolving and stabilizing native Coelenterazine.   The luciferin is shipped as filtered, lyophilized, batch controlled substrate in 500 µg and 1 mg aliquots (1 mg = 2x 500 µg).  10 mgs is packed as regular powder in one tube.

Advantages

  • pre-aliquoted in small amounts, fresh substrate for every set of experiments
  • long shelf-life (packed under Argon)
  • faster to dissolve due to its fine crystal structure
  • consistent quality between aliquotes
     

pGluc-basic

Map

Sequence
203 pGluc-basic - map
203 pGLuc-basic -1 sequence_mappdf-icon 203 pGLuc-basic -1 sequence_mapview-icon

Application

Gaussia luciferase is used as a reporter in conjunction with CRISPR to investigate the function of noncoding RNAs (ncRNA) : Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display.  Shechner DM, Hacisuleyman E, Younger ST, Rinn JL. July 2015. PubMed

pUC18 h- R.M. GFP

Sequence

104 pUC18 humanized R_M_GFP sequencepdf-icon Sequenceview-icon

Renilla Mulleri Luciferase

RmLuc Lot#K2217
Amino Acid sequence:

MTSKVYDPELRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENAVIFLHGNAASSYLWRHVVPHVEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTEWFKHLNLPKKIIFVGHDWGACLAFHYCYEHQDRIKAVVHAESVVDVIESWDEWPDIEEDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREIPLVKGGKPDVVEIVRNYNAYLRASHDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVKGLHFSQEDAPDEMGNYIKSFVERVLKNEQ

v-Coelenterazine-SOL (in vitro)

v-CTZ_600dpi

Advantages of water-soluble Coelenterazine

  • convenient to use, no pre-dissolving of h CTZ in alcohol necessary
  • can be used with different buffers for assay optimization 

Coelenterazine F

Package Contents: 500 ugs  – 1 vial of 500 ugs lyophilized CTZ-f 1 mgs – 2 vials of 500 ugs lyophilized CTZ-f 5 mgs – 1 vial of 5 mgs lyophilized CTZ-f 10 mgs – 2 vials of 5 mgs lyophilized CTZ-f  

Advantages

  • pre-aliquoted in small amounts, fresh substrate for every set of experiments
  • long shelf-life (packed under Argon)
  • faster to dissolve due to its fine crystal structure
  • consistent quality between aliquotes
  The luciferin is shipped as filtered, lyophilized, batch controlled substrate

pUC18 h- Pt. GFP

Sequence

105 pUC18-humanized-PtGFP Sequencepdf-icon Sequenceview-icon

pUC19 R.M. Luc

Sequence

204 pUC19-RM-Luc sequencepdf-icon Sequenceview-icon

e-Coelenterazine-F

Emission spectrum of eCTZ-F

eCTZ-F is showing a unique emission spectrum in combination with Renilla Luciferase (RLuc), emitting a high energy wide spectrum from 400 to 550 nm. Please see the manual for details. The luciferin is shipped as filtered, lyophilized, batch controlled substrate in 500 µg and 1 mg aliquots (1 mg = 2x 500 µg).  10 mgs is packed as regular powder in one tube.

Advantages

  • pre-aliquoted in small amounts, fresh substrate for every set of experiments
  • long shelf-life (packed under Argon)
  • faster to dissolve due to its fine crystal structure
  • consistent quality between aliquots
   

pCMV-h-Pt. GFP

Map

Sequence
108 pCMV-h-Pt GFP map
108 pCMV-humanized-ptGFP sequence mappdf-icon 108 pCMV-humanized-ptGFP sequence mapview-icon

pUC19 Ple-Luc

Sequence

205 pUC19-Ple-Luc sequencepdf-icon Sequenceview-icon

6h-F-Coelenterazine, C6d-f-Coelenterazine

Package Contents: 500 ugs  – 1 vial of 500 µgs lyophilized 6h-F-CTZ 1 mgs – 2 vials of 500 µgs lyophilized 6h-F-CTZ 5 mgs – 1 vial of 5 mgs 6h-F-lyophilized CTZ 10 mgs – 2 vials of 5 mgs 6h-F-lyophilized CTZ

The advantages of lyophilized luciferins are:

  • pre-aliquoted in small amounts, fresh substrate for every set of experiments
  • long shelf-life (packaged under Argon)
  • faster to dissolve due to its fine crystal structure
  • consistent quality between aliquots
This luciferin is shipped as filtered, lyophilized, batch controlled substrate. Available in different sizes. Please inquire for custom aliquots.

pCMV-Gluc-KDEL

Map

Sequence
206 pCMV-Gluc-KDEL map
206 pCMV-KDEL-GLuc-1 sequence_map(2)pdf-icon 206 pCMV-KDEL-GLuc-1 sequence_map(2)view-icon

Application

Gaussia luciferase is used as a reporter in conjunction with CRISPR to investigate the function of noncoding RNAs (ncRNA) : Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display.  Shechner DM, Hacisuleyman E, Younger ST, Rinn JL. July 2015. PubMed

pCMV-h-RM GFP

Map

Sequence
109 pCMV-h-RM GFP map
109 pCMV-humanized-R_M_GFP sequence_mappdf-icon 109 pCMV-humanized-R_M_GFP sequence_mapview-icon