All Products (71)
NanoFuel-400a Solvent
CAT#397 NanoFuel-400a Solvent
NanoFuel®-400a Solvent is NanoLight’s solution to the problem associated with the solubility of the highly hydrophobic Coelenterazine 400a.
Coelenterazine-400a (DeepBlueC™) can be dissolved up to a concentration of 2.5 mg/ml.
NanoFuel-400a Solvent also guarantees Coelenterazine 400a stability if store at -20°C for a few weeks or at -80°C for up to one year.
- Details & Info
- Protocols & Data
- Related Products
NanoKAZ Luciferase Protein (PBS)
CAT#3815 NanoKAZ Luciferase Protein (PBS)
NanoKAZ Luciferase in PBS, an Oplophorus Luciferase mutant is one of the smallest and brightest luciferases. This recombinant protein was affinity purified to give it a maximum specific activity. Buffered in PBS for convenient linkage chemistry where Tris (or other primary amines) would interfere.
Patent US 9,315,783 and US 9,404,145.
- Details & Info
- Protocols & Data
- Related Products
Dry Ice
CAT# Dry Ice
pUC19 Aequorin Photoprotein
CAT#106 pUC19 Aequorin Photoprotein
pUC19 Aequorin Photoprotein Vector
- Details & Info
Ice Pack
CAT# Ice Pack
Used to cool products during transit.
- Details & Info
5x Universal Lysis Buffer
CAT#333 5x Universal Lysis Buffer
- Details & Info
NLuc FLASH Assay
CAT#324 NLuc FLASH Assay
NanoFuel® FLASH Assay for Oplophorus Luciferases
NanoFuel FLASH assay buffer is an alternative for assays that are using substrates like native Coelenterazine or Coeleterazine analogs to achieve even higher bioluminescent readouts with Oplophorus luciferases like NanoLuc® or NanoKAZ.
- Details & Info
- Protocols & Data
- Related Products
Gaussia Luciferase-Streptavidin
CAT#371 Gaussia Luciferase-Streptavidin
Gaussia(M2)-Luciferase-Streptavidin is a conjugate of one of the brightest luciferases and a protein with the highest known affinity in nature. This fusion protein can be used to detect trace amounts of biotinylated protein, DNA, or other biotinylated bio-molecules.
- Details & Info
- Protocols & Data
- Related Products
NanoKAZ Luciferase Protein
CAT#381 NanoKAZ Luciferase Protein
NanoKAZ Luciferase, an Oplophorus Luciferase mutant is one of the smallest and brightest luciferases. This recombinant protein was affinity purified to give it a maximum specific activity. Patent US 9,315,783 and US 9,404,145.
- Details & Info
- Protocols & Data
- Related Products
v-Coelenterazine-SOL (in vitro)
CAT#3702 v-Coelenterazine-SOL (in vitro)
v-Coelenterazine is an analogue of Coelenterazine that causes a shift in the emission-spectrum to longer wavelengths (515nm and above). Renilla Luciferase is the preferred catalyst for this compound. For more details see here.
- Details & Info
- Protocols & Data
NLuc GLOW Assay
CAT#325 NLuc GLOW Assay
NanoFuel® GLOW Assay for Oplophorus Luciferases
Introducing a new GLOW Assay kit that will work with Oplophorus Luciferase and its variants NanoKAZ.
- Details & Info
- Protocols & Data
- Related Products
Firefly Luciferase Assay Reagent
CAT#318 Firefly Luciferase Assay Reagent
The NanoFuel® Assay kit for Firefly Luciferase kit provides enough buffer for approx. 1000 assays. Optimized buffer conditions will give you the best signal over a half-life of 45 min. Therefore no injection luminometer is required. This kit can be used for high-throughput screenings due to its steady signal.
- Details & Info
- Protocols & Data
- Related Products
- Publications
h-Coelenterazine-SOL (in vivo)
CAT#3011 h-Coelenterazine-SOL (in vivo)
- Details & Info
- Protocols & Data
- Related Products
- Publications
e-Coelenterazine-F
CAT#346 e-Coelenterazine-F
e-Coelenterazine-F purity >95% is a new Coelenterazine derivative utilized by Renilla luciferase.
We recommend using CAT#399 NanoFuel Solvent for dissolving and stabilizing native Coelenterazine.
- Details & Info
- Protocols & Data
- Related Products
- FAQs
Prolume Purple- SOL (in vitro)
CAT#3692 Prolume Purple- SOL (in vitro)
Methoxy e-Coelenterazine (Prolume Purple) formulated to be water-soluble for in vitro applications with Renilla Luciferase.
Prolume Purple exhibits an up to 13-fold higher luminescence than CTZ400a (“deep blue C”).
- Details & Info
- Protocols & Data
- Related Products
- Publications
Aequorin photoprotein (lyophilized)
CAT#307P Aequorin photoprotein (lyophilized)
A photoprotein originating from the jellyfish Aequorea victoria emits light in the presence of a trace amount of Ca2+ without the requirement of any other cofactor. Aequorin was “charged” with unmodified (native) Coelenterazine and will emit light at 465 nm upon Ca2+ contact.
- Details & Info
- Protocols & Data
- Related Products
NanoFuel-V Solvent
CAT#398 NanoFuel-V Solvent
Designed by NanoLight to increase the solubility of v-Coelenterazine and Methoxy v-Coelenterazine-Methoxy. Both compounds have a very low solubility in Ethanol and Methanol. Using NanoFuel-V solvent gives you v-CTZ concentrations of up to 4 mg/ml.
- Details & Info
- Protocols & Data
- Related Products
- FAQs
v-Coelenterazine
CAT#370 v-Coelenterazine
V-Coelenterazine (V-CTZ) is a synthetic analogue of Coelenterazine with an additional Benzyl ring. This compound has the unique ability to shift the emission spectra of wildtyp Renilla Luciferase, RLuc8 and Green Renilla Luciferase to longer wavelengths.
V-Coelenterazine is particular difficult to get into solution. We recommend using CAT#398 NanoFuel-V Solvent for dissolving and stabilizing v-Coelenterazine.
- Details & Info
- Protocols & Data
- Related Products
- FAQs
- Publications
Prolume Purple Sampler
CAT#360 Prolume Purple Sampler
Nanolight™ Technologies patented methoxy Coelenterazine compounds are now available as a comprehensive sampler kit.
Nanolight Technology is pleased to introduce these newly developed coelenterazine compounds. Your feedback is appreciated, if you find any additional luciferases or luciferase mutants that work with these compounds, please contact us at info@prolume.com.
- Details & Info
- Related Products
Prolume Purple V
CAT#361 Prolume Purple V
Methoxy-v-Coelenterazine-Methoxy (Me-O-v-CTZ-O-Me) is a new synthetic analogue of v-Coelenterazine with two additional methoxy groups. So far we were not able to determine a luciferase that is working with this unique compound. Activities with RLuc8, Rluc3 and Green Renilla Luciferase are very low.
Nanolight™ Technologies developed Prolume Purple V. (US Patent 2013/057660).
- Details & Info
- Protocols & Data
- Related Products
Prolume Purple IV
CAT#363 Prolume Purple IV
- Details & Info
- Protocols & Data
- Related Products
Prolume Purple III
CAT#365 Prolume Purple III
Methoxy-eCoelenterazine-F (Me-O-eCTZ-F) is a new synthetic analogue of e-Coelenterazine-F with an additional methoxy groups emitting at 410nm with Green Renilla Luciferase. Nanolight™ Technologies developed Prolume Purple III. (US Patent 2013/057660)
If you are using RLuc8 or wildtyp Renilla Luc please use Prolume Purple (Cat.# 369).
- Details & Info
- Protocols & Data
- Related Products
Prolume Purple II
CAT#367 Prolume Purple II
- Details & Info
- Protocols & Data
- Related Products
Prolume Purple
CAT#369 Prolume Purple
Prolume Purple or methoxy e-Coelenterazine (Me-O-e-CTZ) is a new synthetic analogue of e-Coelenterazine with an additional methoxy group quenching the emission maximum from 475 nm to 405 nm. Nanolight™ Technologies developed Prolume Purple. (US Patent 2013/057660)
Prolume Purple exhibits an up to 13-fold higher luminescence than CTZ400a (“deep blue C”).
- Details & Info
- Protocols & Data
- Related Products
- Publications
e-Coelenterazine-INJ
CAT#355-INJ e-Coelenterazine-INJ
e-CTZ is now available in sterile injection vials with a low retention volume. Each kit is suppled with two ‘Inject-A-Lume’ vials of 500 ug e-CTZ and 500 ul of steril ‘Fuel-Inject’ diluent. It was reported that this analogue enhances the signal by two-fold in Renilla Luciferase in vivo studies.
- Details & Info
- Protocols & Data
- Related Products
h-Coelenterazine-SOL (in vitro)
CAT#3012 h-Coelenterazine-SOL (in vitro)
A water-soluble form for in vitro applications.
For additional convenience h-Coelenterazine is now available in a water-soluble form aliquoted in tubes that will give you a 50 µM solution by adding 10 ml of buffer.
- Details & Info
- Related Products
Coelenterazine-SOL (in vitro)
CAT#3032 Coelenterazine-SOL (in vitro)
Native Coelenterazine in a water-soluble form for in vitro applications with Gaussia Luciferase.
For additional convenience native Coelenterazine is now available in a water-soluble form aliquoted in tubes that will give you a 50 µM solution by adding 10 ml of buffer.
- Details & Info
- Protocols & Data
Coelenterazine-SOL (in vivo)
CAT#3031 Coelenterazine-SOL (in vivo)
- Details & Info
- Protocols & Data
- Related Products
- Publications
Aequorin photoprotein (liquid)
CAT#307L Aequorin photoprotein (liquid)
A photoprotein originating from the jellyfish Aequorea victoria emits light in the presence of a trace amount of Ca2+ without the requirement of any other cofactor. Aequorin was “charged” with unmodified (native) Coelenterazine and will emit light at 465 nm upon Ca2+ contact.
- Details & Info
- Protocols & Data
- Related Products
e-Coelenterazine
CAT#355 e-Coelenterazine
e-Coelenterazine or Coelenterazine-E (e-CTZ) is a synthetic analogue of native Coelenterazine with an additional ethyl group, forming an additional ring system. First described by Shimomura in 1989.
Renilla muelleri Luciferase shows an increase of 750% in initial intensity and 137% in total light using e-Coelenterazine compared to native Coelenterazine.
- Details & Info
- Protocols & Data
- Related Products
- FAQs
- Publications
Coenzyme A, free acid
CAT#309 Coenzyme A, free acid
Coenzyme A will prolong the signal of Firefly Luciferase Bioluminescence
- Details & Info
- Protocols & Data
NanoFuel Solvent
CAT#399 NanoFuel Solvent
NanoFuel® Solvent is NanoLight’s solution to the problem associated with the solubility of Coelenterazine and the many new Coelenterazine analogs that have been made available in recent years.
Native Coelenterazine can be dissolved in a 10 times higher concentration compared to other alcohols.
NanoFuel-Solvent also guarantees Coelenterazine stability if store at -20°C for a few weeks or at -80°C for up to one year.
- Details & Info
- Protocols & Data
- FAQs
- Related Products
pUC18 Gluc
CAT#200 pUC18 Gluc
Expressing native Gaussia luciferase without the secretion signal
- Details & Info
By using this product, recipient is legally bound and accepts the Terms and Conditions.
Coelenterazine-INJ
CAT#303-INJ Coelenterazine-INJ
Injectable native Coelenterazine, highly pure (99.7%) and optimized for in vivo research.
- Details & Info
- Protocols & Data
- Related Products
Gaussia Luciferase Protein
CAT#321 Gaussia Luciferase Protein
One of the smallest and brightest cloned luciferases.
- Details & Info
- Protocols & Data
- Related Products
- Publications
GLuc GLOW Assay
CAT#320 GLuc GLOW Assay
Introducing a new one-step GLOW Assay kit for high-throughput screenings (HTS). This kit includes a buffer that will prolong your Gaussia signal over several hours. The half-life of Gaussia in this buffer system is between 2 to 3 hours.
- Details & Info
- Protocols & Data
- Publications
- Related Products
GLuc FLASH Assay
CAT#319 GLuc FLASH Assay
NanoFuel® FLASH Assay for Gaussia Luciferase
The NanoFuel® FLASH Assay kit provides enough buffer for approx. 1000 assays and performs best with a luminometer equipped with an injection port. For assays requiring a longer lasting luminescence (e.g. high-throughput screenings) or if sensitivity is not an issue we recommend our NanoFuel GLOW Assay kit (Cat. #320).
- Details & Info
- Package Info & Data
- Publications
- Related Products
Renilla Reniformis GFP
CAT#316 Renilla Reniformis GFP
Highly purified single band on SDS gels, very active and bright GFP. Has much higher absortion and 495nm excitation, with 3X the fluorescence of eGFP or Aequoia GFP. Emission at 508nm. For best performance should be used with proper filter set.
- Protocols & Data
Ptilosarcus Green Fluorescent protein
CAT#315 Ptilosarcus Green Fluorescent protein
Highly purified single band on SDS gels, very active and bright GFP. Has much higher absortion and 495nm excitation, with 3X the fluorescence of eGFP or Aequoia GFP. Emission at 508nm. For best performance should be used with proper filter set.
- Protocols & Data
Renilla Mulleri Luciferase
CAT#312 Renilla Mulleri Luciferase
95% purity (recombinant). Supplied as lyophilized powder.
ABS280 of 0.63 = 1.0 mg RM Luc
- Details & Info
- Protocols & Data
Coelenteramine
CAT#300 Coelenteramine
Coelenteramine, 97%
A predominant precursor of most Coelenterazine analogs and native Coelenterazine.
- Details & Info
- Protocols & Data
Coelenteramide
CAT#350 Coelenteramide
Natural marine luciferase oxidation amide product of Coelenterazine
- Details & Info
- Protocols & Data
Coelenterazine F
CAT#345 Coelenterazine F
Coelenterazine F is the preferred substrate for Aequorin
We recommend using CAT#399 NanoFuel Solvent for dissolving and stabilizing native Coelenterazine.
- Details & Info
- Protocols & Data
- Related Products
Coelenterazine 400a
CAT#340 Coelenterazine 400a
A substrate for Renilla luciferases (Rluc) but not Gaussia luciferase. It is used for real-time detection of calcium ions, gene reporter assays and a favored substrate for BRET. This derivative has an emission maximum around 400 nm and therefore minimal interference with the emission of the GFP acceptor.
Coelenterazine 400a is also know under the Perkin Elmer trademark DeepBlueC™.
We recommend using CAT#397 NanoFuel-400a Solvent for dissolving and stabilizing Coelenterazine 400a.
- Details & Info
- Protocols & Data
- Related Products
- FAQs
- Publications
Coelenterazine native
CAT#303 Coelenterazine native
Coelenterazine Free Base purity >95%
The most abundant form of Coelenterazine found in nature.
Native Coelenterazine is the preferred substrate for Gaussia luciferase.
We recommend using CAT#399 NanoFuel Solvent for dissolving and stabilizing native Coelenterazine.
- Details & Info
- Protocols & Data
- Related Products
- FAQs
- Publications
Firefly Luciferin Free Acid
CAT#306 Firefly Luciferin Free Acid
Firefly D-Luciferin Free Acid >99% (Photinus pyralis, pyrophorus, and Elateridea substrate luciferins)
This is the FREE ACID and not a water soluble salt, such as the Potassium or Sodium salts. This requires reconstitution using alkaline buffers.
- Details & Info
- Protocols & Data
- Related Products
Cypridina Luciferin
CAT#305 Cypridina Luciferin
Known also as Vargula Luciferin or Vargulin
- Details & Info
- Protocols & Data
h-Coelenterazine-INJ
CAT#301-INJ h-Coelenterazine-INJ
Injectable h-Coelenterazine, highly pure (99%) and optimized for in vivo research.
- Details & Info
- Protocols & Data
- Related Products
Coelenterazine h
CAT#301 Coelenterazine h
A 2-deoxy derivative of native Coelenterazine.
Up to 20-fold higher initial light-output than native Coelenterazine with Apoaequorin.
Reduced but longer lasting kinetic with Renilla luciferase.
We recommend using CAT#399 NanoFuel Solvent for dissolving and stabilizing native Coelenterazine.
- Details & Info
- Protocols & Data
- Related Products
- FAQs
- Publications
Polyclonal Antisera RR GFP
CAT#403P Polyclonal Antisera RR GFP
Polyclonal Rabbit Antibody to Renilla Reniformis GFP Titer>1:10,000 250 µl
- Protocols & Data
Polyclonal Antisera Pt. GFP
CAT#402P Polyclonal Antisera Pt. GFP
Polyclonal Rabbit Antibody to Ptilosarcus GFP Titer>1:10,000 250 µl.
- Protocols & Data
Monoclonal Anti-Gluc
CAT#401M Monoclonal Anti-Gluc
Monoclonal mouse Antibody to Gaussia Luciferase. Titer>1:10,000, 250 µl
- Details & Info
- Related Products
Polyclonal Antisera Gluc
CAT#401P Polyclonal Antisera Gluc
Polyclonal Rabbit Antibody to Gaussia Luciferase Titer>1:10,000. 250 µl
- Protocols & Data
- Related Products
pART27 - ptGFP
CAT#121 pART27 - ptGFP
A binary vector (5 µg dry) with the whole gene cassette including Ptilosarcus GFP-gene inserted via NotI for efficient insertion of the ptGFP-gene cassette into plants’ genome via agrobacterium-mediated transformation.
- Details & Info
By using this product, recipient is legally bound and accepts the Terms and Conditions.
pART7-Pt-GFP
CAT#120 pART7-Pt-GFP
pART7 Ptilosarcus Green Fluorescent Protein (5 µg dry) Novel pH indicating “pHluorin”.
- Details & Info
By using this product, recipient is legally bound and accepts the Terms and Conditions.
pCMV-h-RM GFP
CAT#109 pCMV-h-RM GFP
Humanized Renilla Mulleri Green Fluorescent Protein
- Details & Info
By using this product, recipient is legally bound and accepts the Terms and Conditions.
pCMV-h-Pt. GFP
CAT#108 pCMV-h-Pt. GFP
Humanized Ptilosarcus Green Fluorescent Protein
- Details & Info
By using this product, recipient is legally bound and accepts the Terms and Conditions.
pUC18 Obelin
CAT#107 pUC18 Obelin
pUC18 Obelin Photoprotein
- Details & Info
pUC18 h- Pt. GFP
CAT#105 pUC18 h- Pt. GFP
Humanized Ptilosarcus Green Fluorescent Protein
- Details & Info
By using this product, recipient is legally bound and accepts the Terms and Conditions.
pUC18 h- R.M. GFP
CAT#104 pUC18 h- R.M. GFP
Humanized Renilla Mulleri Green Flrouescent Protein
- Details & Info
By using this product, recipient is legally bound and accepts the Terms and Conditions.
pUC18 Rr. GFP
CAT#103 pUC18 Rr. GFP
Native Renilla Reniformis Green Fluorescent Protein
- Details & Info
By using this product, recipient is legally bound and accepts the Terms and Conditions.
pUC18 Pt. GFP
CAT#102 pUC18 Pt. GFP
Native Ptilosarcus Green Fluorescent Protein
- Details & Info
By using this product, recipient is legally bound and accepts the Terms and Conditions.
pUC19 R.M. GFP
CAT#101 pUC19 R.M. GFP
Native Renilla Mulleri Green Fluorescent protein in pUC19
- Details & Info
pCMV- Gluc-minus SS
CAT#209 pCMV- Gluc-minus SS
Humanized Gaussia luciferase without secretion signal. This positive control vector is very useful in evaluating the efficiency of intracellular transgene expression using Gaussia luciferase as a reporter. Also, this vector is useful for fusing Gluc to a specific protein of interest. This vector has both Ampicillin resistance and Neomycin resistance. Therefore it can be easily propagated in E. coli and can be used to establish stable cell lines expressing Gaussia luciferase.
- Details & Info
By using this product, recipient is legally bound and accepts the Terms and Conditions.
pGLuc-KDEL-Basic
CAT#207 pGLuc-KDEL-Basic
Humanized Gaussia Luciferase, promoterless; designed for insertion of your own promoters, comes with C-terminal KDEL endoplasmic retention signal for intracellular expression.
- Details & Info
By using this product, recipient is legally bound and accepts the Terms and Conditions.
pCMV-Gluc-KDEL
CAT#206 pCMV-Gluc-KDEL
Humanized Gaussia luciferase with C-terminal KDEL endoplasmic reticulum retention signal for intracellular expression. The KDEL sequence is an endoplasmic reticulum retention signal which causes retention of the Gaussia luciferase in the endoplasmic reticulum and results in high levels of intracellular luciferase expression.
- Details & Info
By using this product, recipient is legally bound and accepts the Terms and Conditions.
pUC19 Ple-Luc
CAT#205 pUC19 Ple-Luc
Native Pleuromamma Luciferase
- Details & Info
By using this product, recipient is legally bound and accepts the Terms and Conditions.
pUC19 R.M. Luc
CAT#204 pUC19 R.M. Luc
Native Renilla Mullerei Luciferase
- Details & Info
By using this product, recipient is legally bound and accepts the Terms and Conditions.
pGluc-basic
CAT#203 pGluc-basic
A promoterless vector with a MCS site upstream of the humanized Gaussia luciferase coding sequence (with secretion signal). This vector is designed for promoter analysis and will express secreted Gaussia luciferase.
- Details & Info
By using this product, recipient is legally bound and accepts the Terms and Conditions.
pCMV-Gluc
CAT#202 pCMV-Gluc
Mammalian (human) codon optimized Gaussia luciferase with N-termnal secretion signal. This product can be used as a positive control vector is very useful in evaluating the efficiency of transgene expression using Gaussia luciferase as a reporter. This vector has both Ampicillin resistance and Neomycin resistance. Therefore it can be easily propagated in E. coli and can be used to establish stable cell lines expressing Gaussia luciferase.
- Details & Info
By using this product, recipient is legally bound and accepts the Terms and Conditions.
pUC18 SS-Gluc
CAT#201 pUC18 SS-Gluc
Expressing native Gaussia luciferase with the N-terminal secretion signal
- Details & Info
By using this product, recipient is legally bound and accepts the Terms and Conditions.