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Dry Ice

pUC19 Aequorin Photoprotein

Ice Pack

CAT# Ice Pack

shipping cool

Used to cool products during transit.

5x Universal Lysis Buffer

CAT#333 5x Universal Lysis Buffer

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This buffer provides optimal lysis conditions for cells expressing Firefly, Renilla and Oplophorus (NanoLuc, NanoKAZ) luciferases.
The proprietary formula has stabilizing properties and will enhance the performance of the expressed luciferase.

NLuc FLASH Assay

CAT#324 NLuc FLASH Assay

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NanoFuel® FLASH Assay for Oplophorus Luciferases

NanoFuel FLASH assay buffer is an alternative for assays that are using substrates like native Coelenterazine or Coeleterazine analogs to achieve even higher bioluminescent readouts with Oplophorus luciferases like NanoLuc® or NanoKAZ.

Gaussia Luciferase-Streptavidin

CAT#371 Gaussia Luciferase-Streptavidin

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Gaussia(M2)-Luciferase-Streptavidin is a conjugate of one of the brightest luciferases and a protein with the highest known affinity in nature. This conjugate can be used to detect trace amounts of biotinylated protein, DNA, or other biotinylated bio-molecules.

 

NanoKAZ Luciferase Protein

CAT#381 NanoKAZ Luciferase Protein

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NanoKAZ Luciferase, an Oplophorus Luciferase mutant is one of the smallest and brightest luciferases. This recombinant protein was affinity purified to give it a maximum specific activity. Patent US 9,315,783 and US 9,404,145. 

v-Coelenterazine-SOL (in vitro)

CAT#3702 v-Coelenterazine-SOL (in vitro)

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v-Coelenterazine is an analogue of Coelenterazine that causes a shift in the emission-spectrum to longer wavelengths (515nm and above). Renilla Luciferase is the preferred catalyst for this compound. For more details see here.

NLuc GLOW Assay

CAT#325 NLuc GLOW Assay

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NanoFuel® GLOW Assay for Oplophorus Luciferases

Introducing a new GLOW Assay kit that will work with Oplophorus Luciferase and its variants NanoKAZ. 

Firefly Luciferase Assay Reagent

CAT#318 Firefly Luciferase Assay Reagent

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The NanoFuel® Assay kit for Firefly Luciferase kit provides enough buffer for approx. 1000 assays. Optimized buffer conditions will give you the best signal over a half-life of 45 min. Therefore no injection luminometer is required. This kit can be used for high-throughput screenings due to its steady signal.

h-Coelenterazine-SOL (in vivo)

CAT#3011 h-Coelenterazine-SOL (in vivo)

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Water-soluble h-Coelenterazine is formulated to be water-soluble and safe for in vivo imaging applications with Renilla Luciferase.
 

e-Coelenterazine-F

CAT#346 e-Coelenterazine-F

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e-Coelenterazine-F  purity >95% is a new Coelenterazine derivative utilized by Renilla luciferase.

Prolume Purple- SOL (in vitro)

CAT#3692 Prolume Purple- SOL (in vitro)

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Methoxy e-Coelenterazine (Prolume Purple) formulated to be water-soluble for in vitro applications with Renilla Luciferase.

Prolume Purple exhibits an up to 13-fold higher luminescence than CTZ400a (“deep blue C”).

Aequorin photoprotein (lyophilized)

CAT#307P Aequorin photoprotein (lyophilized)

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A photoprotein originating from the jellyfish Aequorea victoria emits light in the presence of a trace amount of Ca2+ without the requirement of any other cofactor. Aequorin was “charged” with unmodified (native) Coelenterazine and will emit light at 465 nm upon Ca2+ contact.

NanoFuel-V Solvent

CAT#398 NanoFuel-V Solvent

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Designed by NanoLight to increase the solubility of v-Coelenterazine and Methoxy v-Coelenterazine-Methoxy. Both compounds have a very low solubility in Ethanol and Methanol. Using NanoFuel-V solvent gives you v-CTZ concentrations of up to 4 mg/ml.

v-Coelenterazine

CAT#370 v-Coelenterazine

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V-Coelenterazine (V-CTZ) is a synthetic analogue of Coelenterazine with an additional Benzyl ringThis compound has the unique ability to shift the emission spectra of wildtyp Renilla Luciferase, RLuc8 and Green Renilla Luciferase to longer wavelengths.

Prolume Purple Sampler

CAT#360 Prolume Purple Sampler

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Nanolight™ Technologies patented methoxy Coelenterazine compounds are now available as a comprehensive sampler kit. 

Nanolight Technology is pleased to introduce these newly developed coelenterazine compounds. Your feedback is appreciated, if you find any additional luciferases or luciferase mutants that work with these compounds, please contact us at info@prolume.com.

Prolume Purple V

CAT#361 Prolume Purple V

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Methoxy-v-Coelenterazine-Methoxy (Me-O-v-CTZ-O-Me) is a new synthetic analogue of v-Coelenterazine with two additional methoxy groups. So far we were not able to determine a luciferase that is working with this unique compound. Activities with RLuc8, Rluc3 and Green Renilla Luciferase are very low.
Nanolight™ Technologies developed Prolume Purple V. (US Patent 2013/057660).

Prolume Purple IV

CAT#363 Prolume Purple IV

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Prolume Purple IV or Methoxy-Coelenterazine-Iodine (Me-O-CTZ-I) is a new synthetic analogue of Coelenterazine-I with an additional methoxy group. So far we were not able to determine a luciferase that is working with this unique compound. Activity with RLuc8, Rluc3 and Green Renilla Luciferase were very low.
Nanolight™ Technologies developed Prolume Purple IV(US Patent 2013/057660).

Prolume Purple III

CAT#365 Prolume Purple III

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Methoxy-eCoelenterazine-F (Me-O-eCTZ-F) is a new synthetic analogue of e-Coelenterazine-F with an additional methoxy groups emitting at 410nm with Green Renilla Luciferase. Nanolight™ Technologies developed Prolume Purple III. (US Patent 2013/057660)

Prolume Purple III exhibits an up to 10-fold higher luminescence than CTZ400a (“deep blue C”).

If you are using RLuc8 or wildtyp Renilla Luc please use Prolume Purple (Cat.# 369).

Prolume Purple II

CAT#367 Prolume Purple II

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Prolume Purple II or Methoxy-Coelenterazine-Methoxy (Me-O-CTZ-O-Me) is a new synthetic analogue of Coelenterazine with two additional methoxy groups emitting at 400nm with Renilla Luciferase 8 (RLuc8) and Green Renilla Luciferase. Nanolight™ Technologies developed Prolume Purple II (US Patent 2013/057660)
 
Prolume Purple II exhibits an up to 5-fold higher luminescence than CTZ400a (“deep blue C”).

Prolume Purple

CAT#369 Prolume Purple

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Prolume Purple or methoxy e-Coelenterazine (Me-O-e-CTZ) is a new synthetic analogue of e-Coelenterazine with an additional methoxy group quenching the emission maximum from 475 nm to 405 nm. Nanolight™ Technologies developed Prolume Purple. (US Patent 2013/057660)

Prolume Purple exhibits an up to 13-fold higher luminescence than CTZ400a (“deep blue C”).

e-Coelenterazine-INJ

CAT#355-INJ e-Coelenterazine-INJ

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e-CTZ is now available in sterile injection vials with a low retention volume. Each kit is suppled with two ‘Inject-A-Lume’ vials of 500 ug e-CTZ and 500 ul of steril ‘Fuel-Inject’ diluent. It was reported that this analogue enhances the signal by two-fold in Renilla Luciferase in vivo studies.

 

h-Coelenterazine-SOL (in vitro)

CAT#3012 h-Coelenterazine-SOL (in vitro)

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A water-soluble form for in vitro  applications.

For additional convenience h-Coelenterazine is now available in a water-soluble form aliquoted in tubes that will give you a 50 µM solution by adding 10 ml of buffer.

Coelenterazine-SOL (in vitro)

CAT#3032 Coelenterazine-SOL (in vitro)

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Native Coelenterazine in a water-soluble form for in vitro applications with Gaussia Luciferase.

For additional convenience native Coelenterazine is now available in a water-soluble form aliquoted in tubes that will give you a 50 µM solution by adding 10 ml of buffer.

Coelenterazine-SOL (in vivo)

CAT#3031 Coelenterazine-SOL (in vivo)

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Native Coelenterazine formulated to be water-soluble and safe for in vivo imaging applications with Gaussia Luciferase.

Aequorin photoprotein (liquid)

CAT#307L Aequorin photoprotein (liquid)

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A photoprotein originating from the jellyfish Aequorea victoria emits light in the presence of a trace amount of Ca2+ without the requirement of any other cofactor. Aequorin was “charged” with unmodified (native) Coelenterazine and will emit light at 465 nm upon Ca2+ contact.

e-Coelenterazine

CAT#355 e-Coelenterazine

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e-Coelenterazine or Coelenterazine-E (e-CTZ) is a synthetic analogue of native Coelenterazine with an additional ethyl group, forming an additional ring system. First described by Shimomura in 1989.

Renilla muelleri Luciferase shows an increase of 750% in initial intensity and 137% in total light using e-Coelenterazine compared to native Coelenterazine.

Coenzyme A, free acid

CAT#309 Coenzyme A, free acid

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Coenzyme A will prolong the signal of Firefly Luciferase Bioluminescence

NanoFuel Solvent

CAT#399 NanoFuel Solvent

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NanoFuel® Solvent is NanoLight’s solution to the problem associated with the solubility of Coelenterazine and the many new Coelenterazine analogs that have been made available in recent years. 

Native Coelenterazine can be dissolved in a 10 times higher concentration compared to other alcohols.

NanoFuel-Solvent also guarantees Coelenterazine stability if store at -20°C for a few weeks or at -80°C for up to one year.

pUC18 Gluc

CAT#200 pUC18 Gluc

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Expressing native Gaussia luciferase without the secretion signal

By using this product, recipient is legally bound and accepts the Terms and Conditions.

Coelenterazine-INJ

CAT#303-INJ Coelenterazine-INJ

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Injectable native Coelenterazine, highly pure (99.7%) and optimized for in vivo research.

 

Gaussia Luciferase Protein

CAT#321 Gaussia Luciferase Protein

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One of the smallest and brightest cloned luciferases.

GLuc GLOW Assay

CAT#320 GLuc GLOW Assay

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Introducing a new one-step GLOW Assay kit for high-throughput screenings (HTS). This kit includes a buffer that will prolong your Gaussia signal over several hours. The half-life of Gaussia in this buffer system is between 2 to 3 hours.

 

GLuc FLASH Assay

CAT#319 GLuc FLASH Assay

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NanoFuel® FLASH Assay for Gaussia Luciferase

The NanoFuel® FLASH Assay kit provides enough buffer for approx. 1000 assays and performs best with a luminometer equipped with an injection port. For assays requiring a longer lasting luminescence (e.g. high-throughput screenings) or if sensitivity is not an issue we recommend our NanoFuel GLOW Assay kit (Cat. #320).

 

Renilla Reniformis GFP

CAT#316 Renilla Reniformis GFP

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Highly purified single band on SDS gels, very active and bright GFP. Has much higher absortion and 495nm excitation, with 3X the fluorescence of eGFP or Aequoia GFP. Emission at 508nm. For best performance should be used with proper filter set.

Ptilosarcus Green Fluorescent protein

CAT#315 Ptilosarcus Green Fluorescent protein

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Highly purified single band on SDS gels, very active and bright GFP. Has much higher absortion and 495nm excitation, with 3X the fluorescence of eGFP or Aequoia GFP. Emission at 508nm. For best performance should be used with proper filter set.

Renilla Mulleri Luciferase

CAT#312 Renilla Mulleri Luciferase

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95% purity (recombinant). Supplied as lyophilized powder.
ABS280 of 0.63 = 1.0 mg RM Luc

Coelenteramine

CAT#300 Coelenteramine

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Coelenteramine, 97%

A predominant precursor of most Coelenterazine analogs and native Coelenterazine.

Coelenteramide

CAT#350 Coelenteramide

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Natural marine luciferase oxidation amide product of Coelenterazine

Coelenterazine F

CAT#345 Coelenterazine F

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Coelenterazine F is the preferred substrate for Aequorin

Coelenterazine 400a

CAT#340 Coelenterazine 400a

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A substrate for Renilla luciferases (Rluc) but not Gaussia luciferase. It is used for real-time detection of calcium ions, gene reporter assays and a favored substrate for BRET. This derivative has an emission maximum around 400 nm and therefore minimal interference with the emission of the GFP acceptor.
Coelenterazine 400a is also know under the Perkin Elmer trademark DeepBlueC™.

Coelenterazine native

CAT#303 Coelenterazine native

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Coelenterazine Free Base purity >95%

The most abundant form of Coelenterazine found in nature.
Native Coelenterazine is the preferred substrate for Gaussia luciferase.

Firefly Luciferin Free Acid

CAT#306 Firefly Luciferin Free Acid

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Firefly D-Luciferin Free Acid >99% (Photinus pyralis, pyrophorus, and Elateridea substrate luciferins)

This is the FREE ACID and not a water soluble salt, such as the Potassium or Sodium salts. This requires reconstitution using alkaline buffers.

Cypridina Luciferin

CAT#305 Cypridina Luciferin

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Known also as Vargula Luciferin or Vargulin

 

h-Coelenterazine-INJ

CAT#301-INJ h-Coelenterazine-INJ

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Injectable h-Coelenterazine, highly pure (99%) and optimized for in vivo research.

 

Coelenterazine h

CAT#301 Coelenterazine h

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A 2-deoxy derivative of native Coelenterazine.

Up to 20-fold higher initial light-output than native Coelenterazine with Apoaequorin.
Reduced but longer lasting kinetic with Renilla luciferase.

Polyclonal Antisera RR GFP

Polyclonal Antisera Pt. GFP

Monoclonal Antisera Gluc

Polyclonal Antisera Gluc

pART27 - ptGFP

CAT#121 pART27 - ptGFP

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A binary vector (5 µg dry) with the whole gene cassette including Ptilosarcus GFP-gene inserted via NotI for efficient insertion of the ptGFP-gene cassette into plants’ genome via agrobacterium-mediated transformation.

By using this product, recipient is legally bound and accepts the Terms and Conditions.

pART7-Pt-GFP

CAT#120 pART7-Pt-GFP

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pART7 Ptilosarcus Green Fluorescent Protein (5 µg dry) Novel pH indicating “pHluorin”.

By using this product, recipient is legally bound and accepts the Terms and Conditions.

pCMV-h-RM GFP

CAT#109 pCMV-h-RM GFP

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Humanized Renilla Mulleri Green Fluorescent Protein

By using this product, recipient is legally bound and accepts the Terms and Conditions.

pCMV-h-Pt. GFP

CAT#108 pCMV-h-Pt. GFP

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Humanized Ptilosarcus Green Fluorescent Protein

By using this product, recipient is legally bound and accepts the Terms and Conditions.

pUC18 Obelin

pUC18 h- Pt. GFP

pUC18 h- R.M. GFP

pUC18 Rr. GFP

pUC18 Pt. GFP

pUC19 R.M. GFP

CAT#101 pUC19 R.M. GFP

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Native Renilla Mulleri Green Fluorescent protein in pUC19

pCMV- Gluc-minus SS

CAT#209 pCMV- Gluc-minus SS

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Humanized Gaussia luciferase without secretion signal. This positive control vector is very useful in evaluating the efficiency of intracellular transgene expression using Gaussia luciferase as a reporter. Also, this vector is useful for fusing Gluc to a specific protein of interest. This vector has both Ampicillin resistance and Neomycin resistance. Therefore it can be easily propagated in E. coli and can be used to establish stable cell lines expressing Gaussia luciferase.

By using this product, recipient is legally bound and accepts the Terms and Conditions.

pGLuc-KDEL-Basic

CAT#207 pGLuc-KDEL-Basic

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Humanized Gaussia Luciferase, promoterless; designed for insertion of your own promoters, comes with C-terminal KDEL endoplasmic retention signal for intracellular expression.

By using this product, recipient is legally bound and accepts the Terms and Conditions.

pCMV-Gluc-KDEL

CAT#206 pCMV-Gluc-KDEL

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Humanized Gaussia luciferase with C-terminal KDEL endoplasmic reticulum retention signal for intracellular expression. The KDEL sequence is an endoplasmic reticulum retention signal which causes retention of the Gaussia luciferase in the endoplasmic reticulum and results in high levels of intracellular luciferase expression.

By using this product, recipient is legally bound and accepts the Terms and Conditions.

pUC19 Ple-Luc

pUC19 R.M. Luc

pGluc-basic

CAT#203 pGluc-basic

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A promoterless vector with a MCS site upstream of the humanized Gaussia luciferase coding sequence (with secretion signal). This vector is designed for promoter analysis and will express secreted Gaussia luciferase.

By using this product, recipient is legally bound and accepts the Terms and Conditions.

pCMV-Gluc

CAT#202 pCMV-Gluc

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Mammalian (human) codon optimized Gaussia luciferase with N-termnal secretion signal. This product can be used as a positive control vector is very useful in evaluating the efficiency of transgene expression using Gaussia luciferase as a reporter. This vector has both Ampicillin resistance and Neomycin resistance. Therefore it can be easily propagated in E. coli and can be used to establish stable cell lines expressing Gaussia luciferase.

By using this product, recipient is legally bound and accepts the Terms and Conditions.

pUC18 SS-Gluc

CAT#201 pUC18 SS-Gluc

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Expressing native Gaussia luciferase with the N-terminal secretion signal

By using this product, recipient is legally bound and accepts the Terms and Conditions.

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